Download MendeleyThe Structure of CobT from Pyrococcus horikoshii
Cuff, M.E., Evdokimova, E., Mursleen, A., Savchenko, A., Edwards, A., Joachimiak, A.To be published.
3U4G
The Structure of CobT from Pyrococcus horikoshii
- PDB DOI: https://doi.org/10.2210/pdb3U4G/pdb
- Classification: TRANSFERASE
- Organism(s): Pyrococcus horikoshii OT3
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2011-10-07 Released: 2011-12-21
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.90 Å
- R-Value Free: 0.216
- R-Value Work: 0.169
- R-Value Observed: 0.171
wwPDB Validation 3D Report Full Report
This is version 1.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| NaMN:DMB phosphoribosyltransferase | 337 | Pyrococcus horikoshii OT3 | Mutation(s): 0 Gene Names: PH0034 |  | |
UniProt | |||||
Find proteins for O57746 (Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)) Explore O57746 Go to UniProtKB: O57746 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | O57746 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| SO4 Query on SO4 | B [auth A], C [auth A] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
| ACY Query on ACY | D [auth A], E [auth A] | ACETIC ACID C2 H4 O2 QTBSBXVTEAMEQO-UHFFFAOYSA-N |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| MSE Query on MSE | A | L-PEPTIDE LINKING | C5 H11 N O2 Se |  | MET |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.90 Å
- R-Value Free: 0.216
- R-Value Work: 0.169
- R-Value Observed: 0.171
- Space Group: P 64
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 82.751 | α = 90 |
| b = 82.751 | β = 90 |
| c = 84.814 | γ = 120 |
| Software Name | Purpose |
|---|---|
| DENZO | data reduction |
| SCALEPACK | data scaling |
| MLPHARE | phasing |
| DM | phasing |
| REFMAC | refinement |
| PDB_EXTRACT | data extraction |
| SBC-Collect | data collection |
| HKL-3000 | data reduction |
| HKL-3000 | data scaling |
| HKL-3000 | phasing |
| SHELXD | phasing |
| SHELXE | model building |
| SOLVE | phasing |
| RESOLVE | phasing |
| ARP/wARP | model building |
| CCP4 | phasing |
| O | model building |
| Coot | model building |
Entry History
Deposition Data
- Released Date: 2011-12-21 Deposition Author(s): Cuff, M.E., Evdokimova, E., Mursleen, A., Savchenko, A., Edwards, A., Joachimiak, A., Midwest Center for Structural Genomics (MCSG)
Revision History (Full details and data files)
- Version 1.0: 2011-12-21
Type: Initial release - Version 1.1: 2017-11-08
Changes: Refinement description
