Download MendeleyMechanism-based Inactivation by Aromatization of the Transaminase BioA Involved in Biotin Biosynthesis in Mycobaterium tuberculosis.
Shi, C., Geders, T.W., Park, S.W., Wilson, D.J., Boshoff, H.I., Abayomi, O., Barry, C.E., Schnappinger, D., Finzel, B.C., Aldrich, C.C.(2011) J Am Chem Soc 133: 18194-18201
- PubMed: 21988601
- DOI: https://doi.org/10.1021/ja204036t
- Primary Citation of Related Structures:
3TFT, 3TFU - PubMed Abstract:
BioA catalyzes the second step of biotin biosynthesis, and this enzyme represents a potential target to develop new antitubercular agents. Herein we report the design, synthesis, and biochemical characterization of a mechanism-based inhibitor (1) featuring a 3,6-dihydropyrid-2-one heterocycle that covalently modifies the pyridoxal 5'-phosphate (PLP) cofactor of BioA through aromatization. The structure of the PLP adduct was confirmed by MS/MS and X-ray crystallography at 1.94 Å resolution. Inactivation of BioA by 1 was time- and concentration-dependent and protected by substrate. We used a conditional knock-down mutant of M. tuberculosis to demonstrate the antitubercular activity of 1 correlated with BioA expression, and these results provide support for the designed mechanism of action.
Organizational Affiliation:
Academic Health Center, University of Minnesota, Minnesota 55455, United States.
