3SQN

Putative Mga family transcriptional regulator from Enterococcus faecalis

PDB DOI: https://doi.org/10.2210/pdb3SQN/pdb

Classification: Transcription regulator
Organism(s): Enterococcus faecalis
Expression System: Escherichia coli BL21(DE3)
Mutation(s): No

Deposited: 2011-07-05 Released: 2011-07-20
Deposition Author(s): Osipiuk, J., Wu, R., Jedrzejczak, R., Moy, S., Joachimiak, A., Midwest Center for Structural Genomics (MCSG)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.31 Å
R-Value Free: 0.282
R-Value Work: 0.224
R-Value Observed: 0.227

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

Putative Mga family transcriptional regulator from Enterococcus faecalis.

Osipiuk, J., Wu, R., Jedrzejczak, R., Moy, S., Joachimiak, A.

To be published.

Macromolecule Content

Total Structure Weight: 113.7 kDa
Atom Count: 7,103
Modelled Residue Count: 849
Deposited Residue Count: 970
Unique protein chains: 1

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Conserved domain protein	A, B	485	Enterococcus faecalis	Mutation(s): 0 Gene Names: EF_3013
UniProt
Find proteins for Q82ZN7 (Enterococcus faecalis (strain ATCC 700802 / V583)) Explore Q82ZN7 Go to UniProtKB: Q82ZN7
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q82ZN7
Sequence Annotations Expand
Reference Sequence

Small Molecules

Modified Residues 1 Unique
ID	Chains	Type	Formula	2D Diagram	Parent
MSE Query on MSE	A, B	L-PEPTIDE LINKING	C₅ H₁₁ N O₂ Se		MET

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.31 Å
R-Value Free: 0.282
R-Value Work: 0.224
R-Value Observed: 0.227
Space Group: P 1 2₁ 1
Diffraction Data: https://doi.org/10.18430/M33SQN

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 63.803	α = 90
b = 101.759	β = 96.75
c = 89.902	γ = 90

Software Package:

Software Name	Purpose
DENZO	data reduction
SCALEPACK	data scaling
REFMAC	refinement
PDB_EXTRACT	data extraction
SBC-Collect	data collection
HKL-3000	data reduction
HKL-3000	data scaling
PHENIX	phasing

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2011-07-20

Deposition Author(s):

Jedrzejczak, R.

Midwest Center for Structural Genomics (MCSG)

Revision History (Full details and data files)

Version 1.0: 2011-07-20
Type: Initial release
Version 1.1: 2017-11-08
Changes: Refinement description

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.