3SHW

Crystal structure of ZO-1 PDZ3-SH3-Guk supramodule complex with Connexin-45 peptide

PDB DOI: https://doi.org/10.2210/pdb3SHW/pdb

Classification: CELL ADHESION
Organism(s): Homo sapiens
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2011-06-17 Released: 2011-09-28
Deposition Author(s): Yu, J., Pan, L., Chen, J., Yu, H., Zhang, M.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.90 Å
R-Value Free: 0.253
R-Value Work: 0.209
R-Value Observed: 0.211

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

The Structure of the PDZ3-SH3-GuK Tandem of ZO-1 Suggests a Supramodular Organization of the Conserved MAGUK Family Scaffold Core

Pan, L., Chen, J., Yu, J., Yu, H., Zhang, M.

To be published.

Macromolecule Content

Total Structure Weight: 54.06 kDa
Atom Count: 2,759
Modelled Residue Count: 342
Deposited Residue Count: 478
Unique protein chains: 2

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Tight junction protein ZO-1	A	468	Homo sapiens	Mutation(s): 0
UniProt & NIH Common Fund Data Resources
Find proteins for Q07157 (Homo sapiens) Explore Q07157 Go to UniProtKB: Q07157
PHAROS: Q07157 GTEx: ENSG00000104067
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q07157
Sequence Annotations Expand
Reference Sequence

Find similar proteins by: Sequence | 3D Structure

Entity ID: 2
Molecule	Chains	Sequence Length	Organism	Details	Image
Gap junction gamma-1 protein	B	10	N/A	Mutation(s): 0
UniProt & NIH Common Fund Data Resources
Find proteins for P36383 (Homo sapiens) Explore P36383 Go to UniProtKB: P36383
PHAROS: P36383 GTEx: ENSG00000182963
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P36383
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.90 Å
R-Value Free: 0.253
R-Value Work: 0.209
R-Value Observed: 0.211
Space Group: P 2₁ 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 49.005	α = 90
b = 86.955	β = 90
c = 141.401	γ = 90

Software Package:

Software Name	Purpose
HKL-2000	data collection
PHASES	phasing
REFMAC	refinement
HKL-2000	data reduction
HKL-2000	data scaling

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2011-09-28

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2011-09-28
Type: Initial release
Version 1.1: 2024-03-20
Changes: Data collection, Database references

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.