3SER
Zn-mediated Polymer of Maltose-binding Protein K26H/K30H by Synthetic Symmetrization
- PDB DOI: https://doi.org/10.2210/pdb3SER/pdb
- Classification: SUGAR BINDING PROTEIN
- Organism(s): Escherichia coli K-12
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2011-06-11 Released: 2011-09-21
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.35 Å
- R-Value Free: 0.222
- R-Value Work: 0.181
- R-Value Observed: 0.183
wwPDB Validation 3D Report Full Report
This is version 2.1 of the entry. See complete history.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Maltose-binding periplasmic protein | A, B [auth C] | 372 | Escherichia coli K-12 | Mutation(s): 2 Gene Names: b4034, JW3994, malE |  |
UniProt | |||||
Find proteins for P0AEX9 (Escherichia coli (strain K12)) Explore P0AEX9 Go to UniProtKB: P0AEX9 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P0AEX9 | ||||
Sequence AnnotationsExpand | |||||
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Oligosaccharides
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| ZN Query on ZN | G [auth C], H [auth C] | ZINC ION Zn PTFCDOFLOPIGGS-UHFFFAOYSA-N |  | ||
| CA Query on CA | F [auth A] | CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N |  | ||
| CL Query on CL | E [auth A], I [auth C], J [auth C] | CHLORIDE ION Cl VEXZGXHMUGYJMC-UHFFFAOYSA-M |  | ||
Biologically Interesting Molecules (External Reference) 1 Unique
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| ID | Chains | Name | Type/Class | 2D Diagram | 3D Interactions |
| PRD_900001 Query on PRD_900001 | C [auth B], D | alpha-maltose | Oligosaccharide / Nutrient |  | |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.35 Å
- R-Value Free: 0.222
- R-Value Work: 0.181
- R-Value Observed: 0.183
- Space Group: P 1 21 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 57.7 | α = 90 |
| b = 116.37 | β = 99.47 |
| c = 65.04 | γ = 90 |
| Software Name | Purpose |
|---|---|
| XSCALE | data scaling |
| PHASER | phasing |
| PHENIX | refinement |
| PDB_EXTRACT | data extraction |
Entry History
Deposition Data
- Released Date: 2011-09-21 Deposition Author(s): Zhao, M., Soriaga, A.B., Laganowsky, A., Sawaya, M.R., Cascio, D., Yeates, T.O.
Revision History (Full details and data files)
- Version 1.0: 2011-09-21
Type: Initial release - Version 1.1: 2011-11-02
Changes: Database references - Version 1.2: 2017-10-11
Changes: Data collection - Version 2.0: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Atomic model, Data collection, Database references, Derived calculations, Non-polymer description, Structure summary - Version 2.1: 2024-02-28
Changes: Data collection, Database references, Structure summary
