3S88

Crystal structure of Sudan Ebolavirus Glycoprotein (strain Gulu) bound to 16F6


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 3.35 Å
  • R-Value Free: 0.278 
  • R-Value Work: 0.222 
  • R-Value Observed: 0.222 

wwPDB Validation   3D Report Full Report


This is version 2.1 of the entry. See complete history


Literature

A shared structural solution for neutralizing ebolaviruses.

Dias, J.M.Kuehne, A.I.Abelson, D.M.Bale, S.Wong, A.C.Halfmann, P.Muhammad, M.A.Fusco, M.L.Zak, S.E.Kang, E.Kawaoka, Y.Chandran, K.Dye, J.M.Saphire, E.O.

(2011) Nat Struct Mol Biol 18: 1424-1427

  • DOI: https://doi.org/10.1038/nsmb.2150
  • Primary Citation of Related Structures:  
    3S88

  • PubMed Abstract: 

    Sudan virus (genus Ebolavirus) is lethal, yet no monoclonal antibody is known to neutralize it. We here describe antibody 16F6 that neutralizes Sudan virus and present its structure bound to the trimeric viral glycoprotein. Unexpectedly, the 16F6 epitope overlaps that of KZ52, the only other antibody against the GP(1,2) core to be visualized to date. Furthermore, both antibodies against this crucial epitope bridging GP1-GP2 neutralize at a post-internalization step--probably fusion.


  • Organizational Affiliation

    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California, USA.


Macromolecules
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Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
16F6 - Heavy chainA [auth H]220Mus musculusMutation(s): 0 
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
Sequence Annotations
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  • Reference Sequence
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Entity ID: 2
MoleculeChains Sequence LengthOrganismDetailsImage
Envelope glycoproteinB [auth I]298Sudan ebolavirusMutation(s): 0 
Gene Names: GP
UniProt
Find proteins for Q7T9D9 (Sudan ebolavirus (strain Human/Uganda/Gulu/2000))
Explore Q7T9D9 
Go to UniProtKB:  Q7T9D9
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Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupQ7T9D9
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  • Reference Sequence
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Entity ID: 3
MoleculeChains Sequence LengthOrganismDetailsImage
Envelope glycoproteinC [auth J]167Sudan ebolavirusMutation(s): 2 
Gene Names: GP
UniProt
Find proteins for Q7T9D9 (Sudan ebolavirus (strain Human/Uganda/Gulu/2000))
Explore Q7T9D9 
Go to UniProtKB:  Q7T9D9
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupQ7T9D9
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  • Reference Sequence
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Entity ID: 4
MoleculeChains Sequence LengthOrganismDetailsImage
16F6 - Light chainD [auth L]212Mus musculusMutation(s): 0 
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  • Reference Sequence
Oligosaccharides

Help

Entity ID: 5
MoleculeChains Length2D Diagram Glycosylation3D Interactions
2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranoseE [auth A],
F [auth B]
2N-Glycosylation
Glycosylation Resources
GlyTouCan:  G42666HT
GlyCosmos:  G42666HT
GlyGen:  G42666HT
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 3.35 Å
  • R-Value Free: 0.278 
  • R-Value Work: 0.222 
  • R-Value Observed: 0.222 
  • Space Group: I 2 3
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 193.591α = 90
b = 193.591β = 90
c = 193.591γ = 90
Software Package:
Software NamePurpose
ADSCdata collection
PHASERphasing
REFMACrefinement
HKL-2000data reduction
HKL-2000data scaling

Structure Validation

View Full Validation Report



Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2011-12-07
    Type: Initial release
  • Version 1.1: 2011-12-21
    Changes: Database references
  • Version 2.0: 2020-07-29
    Type: Remediation
    Reason: Carbohydrate remediation
    Changes: Atomic model, Data collection, Database references, Derived calculations, Structure summary
  • Version 2.1: 2023-09-13
    Changes: Data collection, Database references, Refinement description, Structure summary