3PJQ
Trypanosoma cruzi trans-sialidase-like inactive isoform (including the natural mutation Tyr342His) in complex with lactose
- PDB DOI: https://doi.org/10.2210/pdb3PJQ/pdb
- Classification: SUGAR BINDING PROTEIN
- Organism(s): Trypanosoma cruzi
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2010-11-10 Released: 2011-06-08
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.10 Å
- R-Value Free: 0.247
- R-Value Work: 0.186
- R-Value Observed: 0.189
wwPDB Validation 3D Report Full Report
This is version 2.0 of the entry. See complete history.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Trans-sialidase | 648 | Trypanosoma cruzi | Mutation(s): 7 EC: 3.2.1.18 |  | |
UniProt | |||||
Find proteins for Q26966 (Trypanosoma cruzi) Explore Q26966 Go to UniProtKB: Q26966 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q26966 | ||||
Sequence AnnotationsExpand | |||||
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Oligosaccharides
Biologically Interesting Molecules (External Reference) 1 Unique
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| ID | Chains | Name | Type/Class | 2D Diagram | 3D Interactions |
| PRD_900008 Query on PRD_900008 | B | alpha-lactose | Oligosaccharide / Nutrient |  | |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.10 Å
- R-Value Free: 0.247
- R-Value Work: 0.186
- R-Value Observed: 0.189
- Space Group: P 1 21 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 54.391 | α = 90 |
| b = 130.217 | β = 107.9 |
| c = 54.529 | γ = 90 |
| Software Name | Purpose |
|---|---|
| SCALA | data scaling |
| REFMAC | refinement |
| PDB_EXTRACT | data extraction |
| ADSC | data collection |
| MOSFLM | data reduction |
Entry History
Deposition Data
- Released Date: 2011-06-08 Deposition Author(s): Oppezzo, P., Baraibar, M., Obal, G., Pritsch, O., Alzari, P.M., Buschiazzo, A.
Revision History (Full details and data files)
- Version 1.0: 2011-06-08
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance - Version 1.2: 2011-08-24
Changes: Database references - Version 1.3: 2017-11-08
Changes: Refinement description - Version 2.0: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Atomic model, Data collection, Database references, Derived calculations, Non-polymer description, Structure summary
