Download MendeleyBiochemical and structural characterization of a Beta-1,3 1,4-glucanase from Bacillus subtilis 168
Furtado, G.P., Ribeiro, L.F., Santos, C.R., Tonoli, C.C., De Souza, A.R., Oliveira, R.R., Tyago, M.(2011) Process Biochem 46: 1202-1206
3O5S
Crystal Structure of the endo-beta-1,3-1,4 glucanase from Bacillus subtilis (strain 168)
- PDB DOI: https://doi.org/10.2210/pdb3O5S/pdb
- Classification: HYDROLASE
- Organism(s): Bacillus subtilis
- Expression System: Escherichia coli
- Mutation(s): No
- Deposited: 2010-07-28 Released: 2011-07-13
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.20 Å
- R-Value Free: 0.292
- R-Value Work: 0.249
- R-Value Observed: 0.252
wwPDB Validation 3D Report Full Report
This is version 1.4 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Beta-glucanase | 238 | Bacillus subtilis | Mutation(s): 0 Gene Names: bglS, bgl, licS, BSU39070, N15B EC: 3.2.1.73 |  | |
UniProt | |||||
Find proteins for P04957 (Bacillus subtilis (strain 168)) Explore P04957 Go to UniProtKB: P04957 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P04957 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| B3P Query on B3P | C [auth A] | 2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL C11 H26 N2 O6 HHKZCCWKTZRCCL-UHFFFAOYSA-N |  | ||
| CA Query on CA | B [auth A] | CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.20 Å
- R-Value Free: 0.292
- R-Value Work: 0.249
- R-Value Observed: 0.252
- Space Group: P 43 21 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 103.758 | α = 90 |
| b = 103.758 | β = 90 |
| c = 102.097 | γ = 90 |
| Software Name | Purpose |
|---|---|
| MAR345dtb | data collection |
| MOLREP | phasing |
| REFMAC | refinement |
| DENZO | data reduction |
| SCALEPACK | data scaling |
Entry History
Deposition Data
- Released Date: 2011-07-13 Deposition Author(s): Santos, C.R., Tonoli, C.C.C., Souza, A.R., Furtado, G.P., Ribeiro, L.F., Ward, R.J., Murakami, M.T.
Revision History (Full details and data files)
- Version 1.0: 2011-07-13
Type: Initial release - Version 1.1: 2015-02-18
Changes: Database references - Version 1.2: 2017-11-08
Changes: Refinement description - Version 1.3: 2018-01-24
Changes: Database references - Version 1.4: 2024-02-21
Changes: Data collection, Database references, Derived calculations
