3N1C
Crystal structure of the phosphofructokinase-2 from Escherichia coli in complex with fructose-6-phosphate
- PDB DOI: https://doi.org/10.2210/pdb3N1C/pdb
- Classification: TRANSFERASE
- Organism(s): Escherichia coli K-12
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No 
- Deposited: 2010-05-15 Released: 2010-12-08 
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 Å
- R-Value Free: 0.221 
- R-Value Work: 0.180 
- R-Value Observed: 0.182 
This is version 1.3 of the entry. See complete history. 
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
---|---|---|---|---|---|
Molecule | Chains | Sequence Length | Organism | Details | Image |
6-phosphofructokinase isozyme 2 | 309 | Escherichia coli K-12 | Mutation(s): 0  Gene Names: b1723, JW5280, PFK2, pfkB EC: 2.7.1.11 | ||
UniProt | |||||
Find proteins for P06999 (Escherichia coli (strain K12)) Explore P06999  Go to UniProtKB:  P06999 | |||||
Entity Groups   | |||||
Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
UniProt Group | P06999 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
Ligands 1 Unique | |||||
---|---|---|---|---|---|
ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
F6P Query on F6P | E [auth A], F [auth B], G [auth C], H [auth D] | 6-O-phosphono-beta-D-fructofuranose C6 H13 O9 P BGWGXPAPYGQALX-ARQDHWQXSA-N |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 Å
- R-Value Free: 0.221 
- R-Value Work: 0.180 
- R-Value Observed: 0.182 
- Space Group: C 2 2 21
Unit Cell:
Length ( Å ) | Angle ( ˚ ) |
---|---|
a = 68.793 | α = 90 |
b = 153.605 | β = 90 |
c = 223.79 | γ = 90 |
Software Name | Purpose |
---|---|
MOSFLM | data reduction |
SCALA | data scaling |
PHASER | phasing |
PHENIX | refinement |
PDB_EXTRACT | data extraction |
Entry History 
Deposition Data
- Released Date: 2010-12-08  Deposition Author(s): Pereira, H.M., Cabrera, R., Caniuguir, A., Garratt, R.C., Babul, J.
Revision History (Full details and data files)
- Version 1.0: 2010-12-08
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance - Version 1.2: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Advisory, Data collection, Derived calculations, Structure summary - Version 1.3: 2023-09-06
Changes: Data collection, Database references, Refinement description, Structure summary