Download MendeleyMechanism of molecular specificity of RhoGAP domains towards small GTPases of RhoA family.
Utepbergenov, D., Cooper, D.R., Derewenda, U., Somlyo, A.V., Derewenda, Z.S.To be published.
3MSX
Crystal structure of RhoA.GDP.MgF3 in complex with GAP domain of ArhGAP20
- PDB DOI: https://doi.org/10.2210/pdb3MSX/pdb
- Classification: PROTEIN BINDING
- Organism(s): Homo sapiens
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2010-04-29 Released: 2011-03-16
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.65 Å
- R-Value Free: 0.219
- R-Value Work: 0.195
- R-Value Observed: 0.196
This is version 1.4 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Transforming protein RhoA | 180 | Homo sapiens | Mutation(s): 1 Gene Names: ARH12, ARHA, RHO12, RHOA EC: 3.6.5.2 |  | |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for P61586 (Homo sapiens) Explore P61586 Go to UniProtKB: P61586 | |||||
PHAROS: P61586 GTEx: ENSG00000067560 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P61586 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Rho GTPase-activating protein 20 | 201 | Homo sapiens | Mutation(s): 0 Gene Names: ARHGAP20, KIAA1391 |  | |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for Q9P2F6 (Homo sapiens) Explore Q9P2F6 Go to UniProtKB: Q9P2F6 | |||||
PHAROS: Q9P2F6 GTEx: ENSG00000137727 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q9P2F6 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| GDP Query on GDP | C [auth A] | GUANOSINE-5'-DIPHOSPHATE C10 H15 N5 O11 P2 QGWNDRXFNXRZMB-UUOKFMHZSA-N |  | ||
| MGF Query on MGF | E [auth A] | TRIFLUOROMAGNESATE F3 Mg GJOMWUHGUQLOAC-UHFFFAOYSA-K |  | ||
| MG Query on MG | D [auth A] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.65 Å
- R-Value Free: 0.219
- R-Value Work: 0.195
- R-Value Observed: 0.196
- Space Group: C 2 2 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 69.057 | α = 90 |
| b = 88.977 | β = 90 |
| c = 137.725 | γ = 90 |
| Software Name | Purpose |
|---|---|
| DENZO | data reduction |
| SCALEPACK | data scaling |
| AMoRE | phasing |
| REFMAC | refinement |
| PDB_EXTRACT | data extraction |
| SERGUI | data collection |
Entry History
Deposition Data
- Released Date: 2011-03-16 Deposition Author(s): Utepbergenov, D., Cooper, D.R., Derewenda, U., Derewenda, Z.S.
Revision History (Full details and data files)
- Version 1.0: 2011-03-16
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance - Version 1.2: 2017-11-08
Changes: Refinement description - Version 1.3: 2021-10-06
Changes: Database references, Derived calculations - Version 1.4: 2024-02-21
Changes: Data collection
