Download MendeleyStructure of catalase determined by MicroED.
Nannenga, B.L., Shi, D., Hattne, J., Reyes, F.E., Gonen, T.(2014) Elife 3: e03600-e03600
- PubMed: 25303172
- DOI: https://doi.org/10.7554/eLife.03600
- Primary Citation of Related Structures:
3J7B - PubMed Abstract:
MicroED is a recently developed method that uses electron diffraction for structure determination from very small three-dimensional crystals of biological material. Previously we used a series of still diffraction patterns to determine the structure of lysozyme at 2.9 Å resolution with MicroED (Shi et al., 2013). Here we present the structure of bovine liver catalase determined from a single crystal at 3.2 Å resolution by MicroED. The data were collected by continuous rotation of the sample under constant exposure and were processed and refined using standard programs for X-ray crystallography. The ability of MicroED to determine the structure of bovine liver catalase, a protein that has long resisted atomic analysis by traditional electron crystallography, demonstrates the potential of this method for structure determination.
Organizational Affiliation:
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
