Download MendeleyStructural basis of high-fidelity DNA synthesis by yeast DNA polymerase delta
Swan, M.K., Johnson, R.E., Prakash, L., Prakash, S., Aggarwal, A.K.(2009) Nat Struct Mol Biol 16: 979-986
- PubMed: 19718023
- DOI: https://doi.org/10.1038/nsmb.1663
- Primary Citation of Related Structures:
3IAY - PubMed Abstract:
DNA polymerase delta (Pol delta) is a high-fidelity polymerase that has a central role in replication from yeast to humans. We present the crystal structure of the catalytic subunit of yeast Pol delta in ternary complex with a template primer and an incoming nucleotide. The structure, determined at 2.0-A resolution, catches the enzyme in the act of replication, revealing how the polymerase and exonuclease domains are juxtaposed relative to each other and how a correct nucleotide is selected and incorporated. The structure also reveals the 'sensing' interactions near the primer terminus, which signal a switch from the polymerizing to the editing mode. Taken together, the structure provides a chemical basis for the bulk of DNA synthesis in eukaryotic cells and a framework for understanding the effects of cancer-causing mutations in Pol delta.
Organizational Affiliation:
Department of Structural & Chemical Biology, Mount Sinai School of Medicine, New York, New York, USA.
