3F5F
Crystal structure of heparan sulfate 2-O-sulfotransferase from gallus gallus as a maltose binding protein fusion.
- PDB DOI: https://doi.org/10.2210/pdb3F5F/pdb
- Classification: Transport, Transferase
- Organism(s): Escherichia coli K-12, Gallus gallus
- Expression System: Escherichia coli
- Mutation(s): Yes 
- Deposited: 2008-11-03 Released: 2008-12-16 
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.65 Å
- R-Value Free: 0.243 
- R-Value Work: 0.200 
- R-Value Observed: 0.200 
This is version 2.2 of the entry. See complete history. 
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
---|---|---|---|---|---|
Molecule | Chains | Sequence Length | Organism | Details | Image |
Maltose-binding periplasmic protein, Heparan sulfate 2-O-sulfotransferase 1 | 658 | Escherichia coli K-12, Gallus gallus This entity is chimeric | Mutation(s): 1  Gene Names: b4034, HS2ST, HS2ST1, JW3994, malE EC: 2.8.2 | ||
UniProt | |||||
Find proteins for P0AEX9 (Escherichia coli (strain K12)) Explore P0AEX9  Go to UniProtKB:  P0AEX9 | |||||
Find proteins for Q76KB1 (Gallus gallus) Explore Q76KB1  Go to UniProtKB:  Q76KB1 | |||||
Entity Groups   | |||||
Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
UniProt Groups | P0AEX9Q76KB1 | ||||
Sequence AnnotationsExpand | |||||
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Oligosaccharides
Small Molecules
Ligands 1 Unique | |||||
---|---|---|---|---|---|
ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
A3P Query on A3P | C [auth A] | ADENOSINE-3'-5'-DIPHOSPHATE C10 H15 N5 O10 P2 WHTCPDAXWFLDIH-KQYNXXCUSA-N |
Biologically Interesting Molecules (External Reference) 1 Unique
Entity ID: 2 | |||||
---|---|---|---|---|---|
ID | Chains | Name | Type/Class | 2D Diagram | 3D Interactions |
PRD_900001 Query on PRD_900001 | B | alpha-maltose | Oligosaccharide / Nutrient |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.65 Å
- R-Value Free: 0.243 
- R-Value Work: 0.200 
- R-Value Observed: 0.200 
- Space Group: P 63
Unit Cell:
Length ( Å ) | Angle ( ˚ ) |
---|---|
a = 152.647 | α = 90 |
b = 152.647 | β = 90 |
c = 85.686 | γ = 120 |
Software Name | Purpose |
---|---|
StructureStudio | data collection |
MOLREP | phasing |
CNS | refinement |
HKL-2000 | data reduction |
HKL-2000 | data scaling |
Entry History 
Deposition Data
- Released Date: 2008-12-16  Deposition Author(s): Bethea, H.N., Xu, D., Liu, J., Pedersen, L.C.
Revision History (Full details and data files)
- Version 1.0: 2008-12-16
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance - Version 1.2: 2017-08-23
Changes: Refinement description, Source and taxonomy - Version 2.0: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Atomic model, Data collection, Database references, Derived calculations, Structure summary - Version 2.1: 2021-10-20
Changes: Database references, Structure summary - Version 2.2: 2023-09-06
Changes: Data collection, Refinement description