Download MendeleyCrystal structures of choloyl glycine hydrolase from Clostridium perfringens
Rossmann, M., Saenger, W.To be published.
2RLC
Crystal Structure of the Conjugated Bile Acid Hydrolase from Clostridium perfringens in Complex with Reaction Products Glycine and Cholate
- PDB DOI: https://doi.org/10.2210/pdb2RLC/pdb
- Classification: HYDROLASE
- Organism(s): Clostridium perfringens
- Expression System: Escherichia coli
- Mutation(s): No
- Deposited: 2007-10-18 Released: 2009-02-17
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.80 Å
- R-Value Free: 0.227
- R-Value Work: 0.193
- R-Value Observed: 0.195
This is version 1.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Choloylglycine hydrolase | 328 | Clostridium perfringens | Mutation(s): 0 Gene Names: cbh EC: 3.5.1.24 |  | |
UniProt | |||||
Find proteins for P54965 (Clostridium perfringens (strain 13 / Type A)) Explore P54965 Go to UniProtKB: P54965 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P54965 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| CHD Query on CHD | D [auth A] | CHOLIC ACID C24 H40 O5 BHQCQFFYRZLCQQ-OELDTZBJSA-N |  | ||
| SO4 Query on SO4 | B [auth A], C [auth A] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
| GLY Query on GLY | E [auth A] | GLYCINE C2 H5 N O2 DHMQDGOQFOQNFH-UHFFFAOYSA-N |  | ||
| Modified Residues 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| MSE Query on MSE | A | L-PEPTIDE LINKING | C5 H11 N O2 Se |  | MET |
| OCS Query on OCS | A | L-PEPTIDE LINKING | C3 H7 N O5 S |  | CYS |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.80 Å
- R-Value Free: 0.227
- R-Value Work: 0.193
- R-Value Observed: 0.195
- Space Group: P 42 2 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 64.24 | α = 90 |
| b = 64.24 | β = 90 |
| c = 169.9 | γ = 90 |
| Software Name | Purpose |
|---|---|
| XSCALE | data scaling |
| SHARP | phasing |
| REFMAC | refinement |
| PDB_EXTRACT | data extraction |
| ADSC | data collection |
| XDS | data reduction |
Entry History
Deposition Data
- Released Date: 2009-02-17 Deposition Author(s): Rossmann, M., Saenger, W.
Revision History (Full details and data files)
- Version 1.0: 2009-02-17
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance
