2RF6

Crystal Structure of the Vaccinia Virus Dual-Specificity Phosphatase VH1

PDB DOI: https://doi.org/10.2210/pdb2RF6/pdb

Classification: HYDROLASE
Organism(s): Vaccinia virus
Expression System: Escherichia coli
Mutation(s): Yes

Deposited: 2007-09-28 Released: 2008-09-30
Deposition Author(s): Koksal, A.C., Cingolani, G.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.95 Å
R-Value Free: 0.208
R-Value Work: 0.187
R-Value Observed: 0.188

wwPDB Validation 3D Report Full Report

This is version 1.5 of the entry. See complete history.

Literature

Crystal Structure of the Vaccinia Virus Dual-Specificity Phosphatase VH1

Koksal, A.C., Nardozzi, J.D., Cingolani, G.

To be published.

Macromolecule Content

Total Structure Weight: 20.36 kDa
Atom Count: 1,551
Modelled Residue Count: 170
Deposited Residue Count: 176
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Dual specificity protein phosphatase	A	176	Vaccinia virus	Mutation(s): 1 EC: 3.1.3.48 (PDB Primary Data), 3.1.3.16 (PDB Primary Data)
UniProt
Find proteins for P07239 (Vaccinia virus (strain Western Reserve)) Explore P07239 Go to UniProtKB: P07239
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P07239
Sequence Annotations Expand
Reference Sequence

Small Molecules

Ligands 1 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
SO4 Query on SO4 Download Ideal Coordinates CCD File SDF format, chain B [auth A] MOL2 format, chain B [auth A]	B [auth A]	SULFATE ION O₄ S QAOWNCQODCNURD-UHFFFAOYSA-L		Interactions Focus chain B [auth A] Interactions & Density Focus chain B [auth A]

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.95 Å
R-Value Free: 0.208
R-Value Work: 0.187
R-Value Observed: 0.188
Space Group: C 2 2 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 38.638	α = 90
b = 63.994	β = 90
c = 135.428	γ = 90

Software Package:

Software Name	Purpose
REFMAC	refinement
CrysalisPro	data collection
MOSFLM	data reduction
SCALA	data scaling
MOLREP	phasing

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2008-09-30

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2008-09-30
Type: Initial release
Version 1.1: 2011-07-13
Changes: Version format compliance
Version 1.2: 2017-10-25
Changes: Refinement description
Version 1.3: 2018-04-04
Changes: Data collection
Version 1.4: 2021-10-20
Changes: Database references, Derived calculations
Version 1.5: 2023-08-30
Changes: Data collection, Refinement description

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.