2QWT

Crystal structure of the TetR transcription regulatory protein from Mycobacterium vanbaalenii

PDB DOI: https://doi.org/10.2210/pdb2QWT/pdb

Classification: TRANSCRIPTION
Organism(s): Mycolicibacterium vanbaalenii PYR-1
Expression System: Escherichia coli BL21(DE3)
Mutation(s): Yes

Deposited: 2007-08-10 Released: 2007-08-28
Deposition Author(s): Bonanno, J.B., Freeman, J., Bain, K.T., Adams, J., Romero, R., Smith, D., Wasserman, S., Sauder, J.M., Burley, S.K., Almo, S.C., New York SGX Research Center for Structural Genomics (NYSGXRC)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.30 Å
R-Value Free: 0.279
R-Value Work: 0.255
R-Value Observed: 0.256

wwPDB Validation 3D Report Full Report

This is version 1.7 of the entry. See complete history.

Literature

Crystal structure of the TetR transcription regulatory protein from Mycobacterium vanbaalenii.

Bonanno, J.B., Freeman, J., Bain, K.T., Adams, J., Romero, R., Smith, D., Wasserman, S., Sauder, J.M., Burley, S.K., Almo, S.C.

To be published.

Macromolecule Content

Total Structure Weight: 21.04 kDa
Atom Count: 1,248
Modelled Residue Count: 167
Deposited Residue Count: 196
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Transcriptional regulator, TetR family	A	196	Mycolicibacterium vanbaalenii PYR-1	Mutation(s): 2 Gene Names: TetR, Mvan_0763
UniProt
Find proteins for A1T351 (Mycolicibacterium vanbaalenii (strain DSM 7251 / JCM 13017 / BCRC 16820 / KCTC 9966 / NRRL B-24157 / PYR-1)) Explore A1T351 Go to UniProtKB: A1T351
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	A1T351
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.30 Å
R-Value Free: 0.279
R-Value Work: 0.255
R-Value Observed: 0.256
Space Group: P 4₃ 2₁ 2

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 57.561	α = 90
b = 57.561	β = 90
c = 98.32	γ = 90

Software Package:

Software Name	Purpose
SCALA	data scaling
REFMAC	refinement
PDB_EXTRACT	data extraction
MAR345	data collection
MOSFLM	data reduction
SHELXCD	phasing
SHELXE	model building

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2007-08-28

Deposition Author(s):

New York SGX Research Center for Structural Genomics (NYSGXRC)

Revision History (Full details and data files)

Version 1.0: 2007-08-28
Type: Initial release
Version 1.1: 2011-07-13
Changes: Version format compliance
Version 1.2: 2017-10-25
Changes: Refinement description
Version 1.3: 2018-11-14
Changes: Data collection, Structure summary
Version 1.4: 2020-02-26
Changes: Database references, Derived calculations
Version 1.5: 2021-02-03
Changes: Database references, Structure summary
Version 1.6: 2021-10-20
Changes: Database references
Version 1.7: 2024-02-21
Changes: Data collection

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.