2Q74

Mycobacterium tuberculosis SuhB


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.60 Å
  • R-Value Free: 0.245 
  • R-Value Work: 0.223 
  • R-Value Observed: 0.224 

wwPDB Validation   3D Report Full Report


This is version 1.2 of the entry. See complete history


Literature

Dimerization of inositol monophosphatase Mycobacterium tuberculosis SuhB is not constitutive, but induced by binding of the activator Mg2+

Brown, A.K.Meng, G.Ghadbane, H.Scott, D.J.Dover, L.G.Nigou, J.Besra, G.S.Futterer, K.

(2007) BMC Struct Biol 7: 55-55

  • DOI: https://doi.org/10.1186/1472-6807-7-55
  • Primary Citation of Related Structures:  
    2Q74

  • PubMed Abstract: 

    The cell wall of Mycobacterium tuberculosis contains a wide range of phosphatidyl inositol-based glycolipids that play critical structural roles and, in part, govern pathogen-host interactions. Synthesis of phosphatidyl inositol is dependent on free myo-inositol, generated through dephosphorylation of myo-inositol-1-phosphate by inositol monophosphatase (IMPase). Human IMPase, the putative target of lithium therapy, has been studied extensively, but the function of four IMPase-like genes in M. tuberculosis is unclear.


  • Organizational Affiliation

    School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. a.k.brown.1@bham.ac.uk


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Inositol-1-monophosphatase
A, B, C
299Mycobacterium tuberculosis H37RvMutation(s): 0 
Gene Names: suhB
EC: 3.1.3.25
UniProt
Find proteins for P9WKI9 (Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
Explore P9WKI9 
Go to UniProtKB:  P9WKI9
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP9WKI9
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.60 Å
  • R-Value Free: 0.245 
  • R-Value Work: 0.223 
  • R-Value Observed: 0.224 
  • Space Group: C 2 2 21
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 101.4α = 90
b = 185.6β = 90
c = 107.3γ = 90
Software Package:
Software NamePurpose
REFMACrefinement
ADSCdata collection
DENZOdata reduction
SCALEPACKdata scaling
AMoREphasing

Structure Validation

View Full Validation Report



Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2007-10-23
    Type: Initial release
  • Version 1.1: 2011-07-13
    Changes: Advisory, Source and taxonomy, Version format compliance
  • Version 1.2: 2023-08-30
    Changes: Data collection, Database references, Refinement description