2PBO

E27Q mutant of EXO-B-(1,3)-Glucanase from Candida Albicans at 1.85 A

PDB DOI: https://doi.org/10.2210/pdb2PBO/pdb

Classification: HYDROLASE
Organism(s): Candida albicans
Expression System: Saccharomyces cerevisiae
Mutation(s): Yes

Deposited: 2007-03-28 Released: 2008-04-01
Deposition Author(s): Cutfield, S.M., Cutfield, J.F., Patrick, W.M.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.85 Å
R-Value Free: 0.158
R-Value Work: 0.130
R-Value Observed: 0.131

wwPDB Validation 3D Report Full Report

This is version 1.4 of the entry. See complete history.

Literature

Structural and Functional Analysis of Protein:Carbohydrate Interactions at Three Distinct Sites in Candida albicans Exo-beta-1,3-glucanase

Patrick, W.M., Cutfield, S.M., Sharpe, M.L., Brooke, G.S., Ramsay, R.J., Cutfield, J.F.

To be published.

Macromolecule Content

Total Structure Weight: 45.79 kDa
Atom Count: 3,554
Modelled Residue Count: 394
Deposited Residue Count: 400
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Hypothetical protein XOG1	A	400	Candida albicans	Mutation(s): 1 Gene Names: XOG1 EC: 3.2.1.58
UniProt
Find proteins for P29717 (Candida albicans (strain SC5314 / ATCC MYA-2876)) Explore P29717 Go to UniProtKB: P29717
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P29717
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.85 Å
R-Value Free: 0.158
R-Value Work: 0.130
R-Value Observed: 0.131
Space Group: P 2₁ 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 60	α = 90
b = 65.17	β = 90
c = 96.69	γ = 90

Software Package:

Software Name	Purpose
DENZO	data reduction
SCALEPACK	data scaling
REFMAC	refinement
PDB_EXTRACT	data extraction
AMoRE	phasing

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2008-04-01

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2008-04-01
Type: Initial release
Version 1.1: 2011-07-13
Changes: Version format compliance
Version 1.2: 2017-10-18
Changes: Refinement description
Version 1.3: 2021-10-20
Changes: Database references
Version 1.4: 2023-08-30
Changes: Data collection, Refinement description

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.