2OQU

High-pressure cryocooling for capillary sample cryoprotection and diffraction phasing at long wavelengths.

(2007) Acta Crystallogr D Biol Crystallogr 63: 653-659

• PubMed: 17452791 Search on PubMed
• DOI: https://doi.org/10.1107/S0907444907011924
• Primary Citation of Related Structures:  
  2OQN, 2OQU


• PubMed Abstract: 
  

  Crystal cryocooling is usually employed to reduce radiation damage during X-ray crystallography. Recently, a high-pressure cryocooling method has been developed which results in excellent diffraction-quality crystals without the use of penetrative cryoprotectants. Three new developments of the method are presented here: (i) Xe-He high-pressure cryocooling for Xe SAD phasing, (ii) native sulfur SAD phasing and (iii) successful cryopreservation of crystals in thick-walled capillaries without additional cryoprotectants other than the native mother liquor. These developments may be useful for structural solution of proteins without the need for selenomethionine incorporation and for high-throughput protein crystallography.

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Organizational Affiliation: 

Field of Biophysics, Cornell University, Ithaca, NY 14853, USA.