Download MendeleyLigand Protection and Nitric Oxide Interactions with Heme in Nitrophorin 4: Kinetic and Structural Analyses of a Loop Mutant
Amoia, A.M., Montfort, W.R.To be published.
2OFR
1.00 A Crystal Structure Of V36A/D129A/L130A Mutant of Nitrophorin 4 From Rhodnius Prolixus Complexed With Nitric Oxide at pH 5.6
- PDB DOI: https://doi.org/10.2210/pdb2OFR/pdb
- Classification: TRANSPORT PROTEIN
- Organism(s): Rhodnius prolixus
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Deposited: 2007-01-04 Released: 2008-01-08
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.00 Å
- R-Value Free: 0.148
- R-Value Work: 0.126
- R-Value Observed: 0.127
This is version 1.3 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Nitrophorin-4 | A [auth X] | 184 | Rhodnius prolixus | Mutation(s): 3 |  |
UniProt | |||||
Find proteins for Q94734 (Rhodnius prolixus) Explore Q94734 Go to UniProtKB: Q94734 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q94734 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| HEM Query on HEM | C [auth X] | PROTOPORPHYRIN IX CONTAINING FE C34 H32 Fe N4 O4 KABFMIBPWCXCRK-RGGAHWMASA-L |  | ||
| PO4 Query on PO4 | B [auth X] | PHOSPHATE ION O4 P NBIIXXVUZAFLBC-UHFFFAOYSA-K |  | ||
| NO Query on NO | D [auth X] | NITRIC OXIDE N O ODUCDPQEXGNKDN-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.00 Å
- R-Value Free: 0.148
- R-Value Work: 0.126
- R-Value Observed: 0.127
- Space Group: C 1 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 70.191 | α = 90 |
| b = 42.637 | β = 94.12 |
| c = 52.975 | γ = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| CrystalClear | data scaling |
Entry History
Deposition Data
- Released Date: 2008-01-08 Deposition Author(s): Amoia, A.M.
Revision History (Full details and data files)
- Version 1.0: 2008-01-08
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance - Version 1.2: 2021-10-20
Changes: Database references, Derived calculations - Version 1.3: 2023-08-30
Changes: Data collection, Refinement description
