2KPP

Solution NMR structure of Lin0431 protein from Listeria innocua. Northeast Structural Genomics Consortium Target LkR112


Experimental Data Snapshot

  • Method: SOLUTION NMR
  • Conformers Calculated: 100 
  • Conformers Submitted: 20 
  • Selection Criteria: structures with the lowest energy 

wwPDB Validation   3D Report Full Report


This is version 1.3 of the entry. See complete history


Literature

Solution NMR structure of Lin0431 protein from Listeria innocua reveals high structural similarity with domain II of bacterial transcription antitermination protein NusG.

Tang, Y.Xiao, R.Ciccosanti, C.Janjua, H.Lee, D.Y.Everett, J.K.Swapna, G.V.Acton, T.B.Rost, B.Montelione, G.T.

(2010) Proteins 78: 2563-2568

  • DOI: https://doi.org/10.1002/prot.22760
  • Primary Citation of Related Structures:  
    2KPP

  • PubMed Abstract: 

    Lin0431 protein from Listeria innocua (UniProtKB/TrEMBL ID Q92EM7/Q92EM7_LISIN) was selected as a target of the Northeast Structural Genomics Consortium (target ID: LkR112). Here, we present the high-quality NMR solution structure of this protein which is the first representative for a member of DUF1312 domain family. Lin0431 protein exhibits a β-sandwich topology. Four anti-parallel β-strands form one face of the sandwich and the other three anti-parallel β-strands together with a short α-helix form the other face of the sandwich. Structure alignment by Dali reveals an unexpected structural similarity with domain II of NusG from Aquifex aeolicus . Analyses of the electrostatic protein surface potential and searches for protein surface cavities reveal the conserved basic charged surface cavities of both the Lin0431 and domain II of Aae NusG, suggesting they may bind the negatively charged nucleic acids and/or and other binding partners. The high structural similarity and similar surface features, despite the lack of recognizable sequence similarity, between Lin0431 and Aae NusG domain II suggest that the domain II of NusG and DUF1312 domains have a homologous relationship and may share similar biochemical functions.


  • Organizational Affiliation

    Department of Molecular Biology and Biochemistry, Rutgers, Center for Advanced Biotechnology and Medicine, The State University of New Jersey, Piscataway, New Jersey 08854, USA.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Lin0431 protein114Listeria innocuaMutation(s): 0 
Gene Names: lin0431
UniProt
Find proteins for Q92EM7 (Listeria innocua serovar 6a (strain ATCC BAA-680 / CLIP 11262))
Explore Q92EM7 
Go to UniProtKB:  Q92EM7
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupQ92EM7
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: SOLUTION NMR
  • Conformers Calculated: 100 
  • Conformers Submitted: 20 
  • Selection Criteria: structures with the lowest energy 

Structure Validation

View Full Validation Report



Entry History 

Revision History  (Full details and data files)

  • Version 1.0: 2010-02-23
    Type: Initial release
  • Version 1.1: 2011-07-13
    Changes: Version format compliance
  • Version 1.2: 2020-02-26
    Changes: Data collection, Database references, Derived calculations, Other
  • Version 1.3: 2023-06-14
    Changes: Database references, Other