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STRUCTURE OF THE THERMUS THERMOPHILUS 70S RIBOSOME COMPLEXED WITH MRNA, TRNA AND PAROMOMYCIN (PART 2 OF 4). THIS FILE CONTAINS THE 50S SUBUNIT FROM MOLECULE I.
 
 
DOI:10.2210/pdb2j01/pdb   NDB ID: 2J01
2J01
 
Split Entry
The asymmetric unit for this structure is composed of multiple PDB entries:
2J00   2J01   2J02   2J03  
Download All Files   
 
 
 
Primary Citation
spinning wheel
 
 
  •  
    Move Section Molecular Description Hide
    Classification: Ribosome
    Structure Weight: 1429029.23
     
    Molecule:50S RIBOSOMAL PROTEIN L27
    Polymer:1Type:polypeptide(L)Length:85
    Chains:0
    Molecule:50S RIBOSOMAL PROTEIN L28
    Polymer:2Type:polypeptide(L)Length:98
    Chains:1
    Molecule:50S RIBOSOMAL PROTEIN L29
    Polymer:3Type:polypeptide(L)Length:72
    Chains:2
    Molecule:50S RIBOSOMAL PROTEIN L30
    Polymer:4Type:polypeptide(L)Length:60
    Chains:3
    Molecule:50S RIBOSOMAL PROTEIN L31
    Polymer:5Type:polypeptide(L)Length:71
    Chains:4
    Molecule:50S RIBOSOMAL PROTEIN L32
    Polymer:6Type:polypeptide(L)Length:60
    Chains:5
    Molecule:50S RIBOSOMAL PROTEIN L33
    Polymer:7Type:polypeptide(L)Length:54
    Chains:6
    Molecule:50S RIBOSOMAL PROTEIN L34
    Polymer:8Type:polypeptide(L)Length:49
    Chains:7
    Molecule:50S RIBOSOMAL PROTEIN L35
    Polymer:9Type:polypeptide(L)Length:65
    Chains:8
    Molecule:23S RIBOSOMAL RNA
    Polymer:10Type:polyribonucleotideLength:2787
    Chains:A
    Other Details:CHAIN A (23S RNA) HAS E.COLI RESIDUE NUMBERING, BASED ON A STRUCTURAL ALIGNMENT WITH THE CORRESPONDING E. COLI STRUCTURE IN 2AW4
    Molecule:5S RIBOSOMAL RNA
    Polymer:11Type:polyribonucleotideLength:122
    Chains:B
    Molecule:50S RIBOSOMAL PROTEIN L1
    Polymer:12Type:polypeptide(L)Length:229
    Chains:C
    Molecule:50S RIBOSOMAL PROTEIN L2
    Polymer:13Type:polypeptide(L)Length:276
    Chains:D
    Molecule:50S RIBOSOMAL PROTEIN L3
    Polymer:14Type:polypeptide(L)Length:206
    Chains:E
    Molecule:50S RIBOSOMAL PROTEIN L4
    Polymer:15Type:polypeptide(L)Length:210
    Chains:F
    Molecule:50S RIBOSOMAL PROTEIN L5
    Polymer:16Type:polypeptide(L)Length:182
    Chains:G
    Molecule:50S RIBOSOMAL PROTEIN L6
    Polymer:17Type:polypeptide(L)Length:180
    Chains:H
    Molecule:50S RIBOSOMAL PROTEIN L9
    Polymer:18Type:polypeptide(L)Length:148
    Chains:I
    Molecule:50S RIBOSOMAL PROTEIN L13
    Polymer:19Type:polypeptide(L)Length:140
    Chains:N
    Molecule:50S RIBOSOMAL PROTEIN L14
    Polymer:20Type:polypeptide(L)Length:122
    Chains:O
    Molecule:50S RIBOSOMAL PROTEIN L15
    Polymer:21Type:polypeptide(L)Length:150
    Chains:P
    Molecule:50S RIBOSOMAL PROTEIN L16
    Polymer:22Type:polypeptide(L)Length:141
    Chains:Q
    Molecule:50S RIBOSOMAL PROTEIN L17
    Polymer:23Type:polypeptide(L)Length:118
    Chains:R
    Molecule:50S RIBOSOMAL PROTEIN L18
    Polymer:24Type:polypeptide(L)Length:112
    Chains:S
    Molecule:50S RIBOSOMAL PROTEIN L19
    Polymer:25Type:polypeptide(L)Length:146
    Chains:T
    Molecule:50S RIBOSOMAL PROTEIN L20
    Polymer:26Type:polypeptide(L)Length:118
    Chains:U
    Molecule:50S RIBOSOMAL PROTEIN L21
    Polymer:27Type:polypeptide(L)Length:101
    Chains:V
    Other Details:CHAIN V (TRNA FMET) HAS RESIDUE NUMBERING BASED ON THE CANONICAL SEQUENCE OF TRNA PHE
    Molecule:50S RIBOSOMAL PROTEIN L22
    Polymer:28Type:polypeptide(L)Length:113
    Chains:W
    Molecule:50S RIBOSOMAL PROTEIN L23
    Polymer:29Type:polypeptide(L)Length:96
    Chains:X
    Molecule:50S RIBOSOMAL PROTEIN L24
    Polymer:30Type:polypeptide(L)Length:110
    Chains:Y
    Molecule:50S RIBOSOMAL PROTEIN L25
    Polymer:31Type:polypeptide(L)Length:206
    Chains:Z
    Molecule:50S RIBOSOMAL PROTEIN L36
    Polymer:32Type:polypeptide(L)Length:37
    Chains:9
    Molecule:50S RIBOSOMAL PROTEIN L10
    Polymer:33Type:polypeptide(L)Length:173
    Chains:J
    Molecule:50S RIBOSOMAL PROTEIN L11
    Polymer:34Type:polypeptide(L)Length:147
    Chains:K
    Molecule:50S RIBOSOMAL PROTEIN L7
    Polymer:35Type:polypeptide(L)Length:125
    Chains:L, M
     
     
  •  
    Move Section Source Hide
    Polymer: 1
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 2
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 3
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 4
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 5
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 6
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 7
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 8
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 9
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 10
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 11
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 12
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 13
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 14
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 15
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 16
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 17
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 18
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 19
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 20
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 21
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 22
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 23
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 24
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 25
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 26
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 27
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 28
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 29
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 30
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 31
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 32
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 33
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 34
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
    Polymer: 35
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry  
     
     
  •  
    Move Section Related PDB Entries Hide
    Id Details
    1BXY  CRYSTAL STRUCTURE OF RIBOSOMAL PROTEIN L30 FROM THERMUSTHERMOPHILUS AT 1.9 A RESOLUTION : CONFORMATIONALFLEXIBILITY OF THE MOLECULE. 
    1TWV  MODEL STRUCTURE OF THE T. THERMOPHILUS 70S RIBOSOME,50SSUBUNIT OF 70S RIBOSOME. THIS FILE, 1TWV, CONTAINS ONLYMOLECULES OF THE 50S RIBOSOMAL SUBUNIT. THE 30S SUBUNIT ISIN THE PDB FILE 1TWT. 
    2B66  50S RIBOSOMAL SUBUNIT FROM A CRYSTAL STRUCTURE OF RELEASEFACTOR RF1, TRNAS AND MRNA BOUND TO THE RIBOSOME. THISFILE CONTAINS THE 50S SUBUNIT FROM A CRYSTAL STRUCTURE OFRELEASE FACTOR RF1, TRNAS AND MRNA BOUND TO THE RIBOSOMEAND IS DESCRIBED IN REMARK 400 
    2B9N  50S RIBOSOMAL SUBUNIT FROM A CRYSTAL STRUCTURE OF RELEASEFACTOR RF2, TRNAS AND MRNA BOUND TO THE RIBOSOME. THISFILE CONTAINS THE 50S SUBUNIT FROM A CRYSTAL STRUCTURE OFRELEASE FACTOR RF1, TRNAS AND MRNA BOUND TO THE RIBOSOMEAND IS DESCRIBED IN REMARK 400. 
    2B9P  50S RIBOSOMAL SUBUNIT FROM A CRYSTAL STRUCTURE OF THERIBOSOME IN COMPLEX WITH TRNAS AND MRNA WITH A STOP CODONIN THE A -SITE. THIS FILE CONTAINS THE 50S SUBUNIT FROM ACRYSTAL STRUCTURE OF THE RIBOSOME IN COMPLEX WITH TRNASAND MRNA WITH A STOP CODON IN THE A-SITE AND IS DESCRIBEDIN REMARK 400. 
    2J00  STRUCTURE OF THE THERMUS THERMOPHILUS 70S RIBOSOME COMPLEXED WITH MRNA, TRNA AND PAROMOMYCIN 
    2J02  STRUCTURE OF THE THERMUS THERMOPHILUS 70S RIBOSOME COMPLEXED WITH MRNA, TRNA AND PAROMOMYCIN 
    2J03  STRUCTURE OF THE THERMUS THERMOPHILUS 70S RIBOSOME COMPLEXED WITH MRNA, TRNA AND PAROMOMYCIN 
     
     
  •  
    Move Section Derived Data Hide
     
     
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Composite of PDB entries (2J00 and 2J01)
 
 
 
  •  
    Move Section Deposition Summary Hide
    Authors:   Selmer, M.,   Dunham, C.M.,   Murphy, F.V.,   Weixlbaumer, A.,   Petry, S.,   Weir, J.R.,   Kelley, A.C.,   Ramakrishnan, V.

    Deposition:   2006-07-31
    Release:   2006-09-28
    Last Modified (REVDAT):   2009-02-24
     
     
  •  
    Move Section Experimental Details Hide
    Method:   X-RAY DIFFRACTION
    Experimental Data:   Download Structure Factors
    View a histogram of Resolution Resolution[Å]: 2.80
    R-Value: 0.271 (obs.)
    R-Free: 0.313
    Space Group: P 21 21 21
    Unit Cell:
      Length [Å] Angles [°]
    a = 213.32 α = 90.00 
    b = 452.95 β = 90.00 
    c = 631.36 γ = 90.00