2IA2

The crystal structure of a putative transcriptional regulator RHA06195 from Rhodococcus sp. RHA1

PDB DOI: https://doi.org/10.2210/pdb2IA2/pdb

Classification: TRANSCRIPTION
Organism(s): Rhodococcus jostii RHA1
Expression System: Escherichia coli
Mutation(s): Yes

Deposited: 2006-09-06 Released: 2006-10-10
Deposition Author(s): Cymborowski, M.T., Chruszcz, M., Skarina, T., Onopriyenko, O., Savchenko, A., Edwards, A., Joachimiak, A., Minor, W., Midwest Center for Structural Genomics (MCSG)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.10 Å
R-Value Free: 0.223
R-Value Work: 0.186
R-Value Observed: 0.188

wwPDB Validation 3D Report Full Report

This is version 1.4 of the entry. See complete history.

Literature

The crystal structure of a putative transcriptional regulator RHA06195 from Rhodococcus sp. RHA1

Cymborowski, M.T., Chruszcz, M., Skarina, T., Onopriyenko, O., Savchenko, A., Edwards, A., Minor, W.

To be published.

Macromolecule Content

Total Structure Weight: 116.28 kDa
Atom Count: 8,064
Modelled Residue Count: 988
Deposited Residue Count: 1,060
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Putative Transcriptional Regulator	A, B, C, D	265	Rhodococcus jostii RHA1	Mutation(s): 4
UniProt
Find proteins for Q0SH23 (Rhodococcus jostii (strain RHA1)) Explore Q0SH23 Go to UniProtKB: Q0SH23
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q0SH23
Sequence Annotations Expand
Reference Sequence

Small Molecules

Modified Residues 1 Unique
ID	Chains	Type	Formula	2D Diagram	Parent
MSE Query on MSE	A, B, C, D	L-PEPTIDE LINKING	C₅ H₁₁ N O₂ Se		MET

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.10 Å
R-Value Free: 0.223
R-Value Work: 0.186
R-Value Observed: 0.188
Space Group: P 2₁ 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 60.892	α = 90
b = 121.526	β = 90
c = 174.259	γ = 90

Software Package:

Software Name	Purpose
REFMAC	refinement
HKL-2000	data collection
HKL-2000	data reduction
HKL-2000	data scaling
HKL-3000	phasing
SHELXD	phasing
SHELXE	model building
MLPHARE	phasing
DM	phasing
CCP4	phasing
SOLVE	phasing
RESOLVE	phasing
ARP/wARP	model building
Coot	model building
O	model building

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2006-10-10

Deposition Author(s):

Cymborowski, M.T.

Onopriyenko, O.

Midwest Center for Structural Genomics (MCSG)

Revision History (Full details and data files)

Version 1.0: 2006-10-10
Type: Initial release
Version 1.1: 2008-05-01
Changes: Version format compliance
Version 1.2: 2011-07-13
Changes: Source and taxonomy, Version format compliance
Version 1.3: 2011-10-05
Changes: Structure summary
Version 1.4: 2022-04-13
Changes: Database references, Derived calculations, Structure summary

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.