2I9E

Deposited: 2006-09-05 Released: 2006-09-26
Deposition Author(s): Schmidt, A., Scheerer, P., Wessner, H., Hoehne, W., Krauss, N.

Experimental Data Snapshot

wwPDB Validation 3D Report Full Report

This is version 1.5 of the entry. See complete history.

Literature

(2010) Insect Mol Biol 19: 35-48

PubMed Abstract:
A coleopteran triosephosphate isomerase (TIM) from Tenebrio molitor (yellow mealworm beetle) was recombinantly expressed in Escherichia coli and characterized with respect to thermal stability, kinetic parameters and oligomeric state. The enzyme was successfully crystallized and the structure determined by X-ray analysis to 2.0 A resolution. This is the first example of an invertebrate TIM. We compare structural features with known structures of TIMs from microorganisms, plants and vertebrates, and discuss the utility of the Tenebrio TIM sequence, together with several newly sequenced insect TIMs, for molecular phylogenetic analysis.

Organizational Affiliation

Institut für Biochemie, Charité- Universitätsmedizin Berlin, Berlin.

Macromolecule Content

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Triosephosphate isomerase	A, B, C, D	259	Tenebrio molitor	Mutation(s): 3 Gene Names: tpi EC: 5.3.1.1
UniProt
Find proteins for Q8MPF2 (Tenebrio molitor) Explore Q8MPF2 Go to UniProtKB: Q8MPF2
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q8MPF2
Sequence Annotations Expand
Reference Sequence

Small Molecules

Ligands 1 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
TRS Query on TRS Download Ideal Coordinates CCD File SDF format, chain E [auth B] SDF format, chain F [auth B] SDF format, chain G [auth C] SDF format, chain H [auth D] SDF format, chain I [auth D] MOL2 format, chain E [auth B] MOL2 format, chain F [auth B] MOL2 format, chain G [auth C] MOL2 format, chain H [auth D] MOL2 format, chain I [auth D]	E [auth B], F [auth B], G [auth C], H [auth D], I [auth D]	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL C₄ H₁₂ N O₃ LENZDBCJOHFCAS-UHFFFAOYSA-O		Interactions Focus chain E [auth B] Focus chain F [auth B] Focus chain G [auth C] Focus chain H [auth D] Focus chain I [auth D] Interactions & Density Focus chain E [auth B] Focus chain F [auth B] Focus chain G [auth C] Focus chain H [auth D] Focus chain I [auth D]