Download MendeleyThe structure of CynR, a transcriptional regulator, both in the presence and absence of sodium azide, its activator ligand
Singer, A.U., Evdokimova, E., Cuff, M.E., Kagan, O., Edwards, A.M., Joachimiak, A., Savchenko, A.To be published.
2HXR
Structure of the ligand binding domain of E. coli CynR, a transcriptional regulator controlling cyanate metabolism
- PDB DOI: https://doi.org/10.2210/pdb2HXR/pdb
- Classification: TRANSCRIPTION
- Organism(s): Escherichia coli K-12
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Deposited: 2006-08-03 Released: 2006-08-22
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.05 Å
- R-Value Free: 0.256
- R-Value Work: 0.239
- R-Value Observed: 0.239
wwPDB Validation 3D Report Full Report
This is version 1.3 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| HTH-type transcriptional regulator cynR | 238 | Escherichia coli K-12 | Mutation(s): 4 Gene Names: cynR |  | |
UniProt | |||||
Find proteins for P27111 (Escherichia coli (strain K12)) Explore P27111 Go to UniProtKB: P27111 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P27111 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| MSE Query on MSE | A, B | L-PEPTIDE LINKING | C5 H11 N O2 Se |  | MET |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.05 Å
- R-Value Free: 0.256
- R-Value Work: 0.239
- R-Value Observed: 0.239
- Space Group: C 1 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 87.913 | α = 90 |
| b = 98.555 | β = 104.35 |
| c = 62.236 | γ = 90 |
| Software Name | Purpose |
|---|---|
| CNS | refinement |
| HKL-2000 | data reduction |
| HKL-2000 | data scaling |
| SHELXCD | phasing |
| SHELXD | phasing |
| SHELXE | model building |
| MLPHARE | phasing |
Entry History
Deposition Data
- Released Date: 2006-08-22 Deposition Author(s): Singer, A.U., Cuff, M.E., Evdokimova, E., Kagan, O., Joachimiak, A., Edwards, A.M., Savchenko, A., Midwest Center for Structural Genomics (MCSG)
Revision History (Full details and data files)
- Version 1.0: 2006-08-22
Type: Initial release - Version 1.1: 2008-05-01
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Source and taxonomy, Version format compliance - Version 1.3: 2017-10-18
Changes: Refinement description
