2EZX

SOLUTION STRUCTURE OF HUMAN BARRIER-TO-AUTOINTEGRATION FACTOR BAF, NMR, REGULARIZED MEAN STRUCTURE


Experimental Data Snapshot

  • Method: SOLUTION NMR
  • Conformers Calculated: 40 
  • Conformers Submitted: 
  • Selection Criteria: REGULARIZED MEAN STRUCTURE 

wwPDB Validation   3D Report Full Report

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This is version 1.4 of the entry. See complete history


Literature

Solution structure of the cellular factor BAF responsible for protecting retroviral DNA from autointegration.

Cai, M.Huang, Y.Zheng, R.Wei, S.Q.Ghirlando, R.Lee, M.S.Craigie, R.Gronenborn, A.M.Clore, G.M.

(1998) Nat Struct Biol 5: 903-909

  • DOI: https://doi.org/10.1038/2345
  • Primary Citation of Related Structures:  
    2EZX, 2EZY, 2EZZ

  • PubMed Abstract: 

    The solution structure of the human barrier-to-autointegration factor, BAF, a 21,000 Mr dimer, has been solved by NMR, including extensive use of dipolar couplings which provide a priori long range structural information. BAF is a highly evolutionarily conserved DNA binding protein that is responsible for inhibiting autointegration of retroviral DNA, thereby promoting integration of retroviral DNA into the host chromosome. BAF is largely helical, and each subunit is composed of five helices. The dimer is elongated in shape and the dimer interface comprises principally hydrophobic contacts supplemented by a single salt bridge. Despite the absence of any sequence similarity to any other known protein family, the topology of helices 3-5 is similar to that of a number of DNA binding proteins, with helices 4 and 5 constituting a helix-turn-helix motif. A model for the interaction of BAF with DNA that is consistent with structural and mutagenesis data is proposed.


  • Organizational Affiliation

    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0520, USA.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
BARRIER-TO-AUTOINTEGRATION FACTOR
A, B
89Homo sapiensMutation(s): 0 
UniProt & NIH Common Fund Data Resources
Find proteins for O75531 (Homo sapiens)
Explore O75531 
Go to UniProtKB:  O75531
PHAROS:  O75531
GTEx:  ENSG00000175334 
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupO75531
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: SOLUTION NMR
  • Conformers Calculated: 40 
  • Conformers Submitted: 
  • Selection Criteria: REGULARIZED MEAN STRUCTURE 

Structure Validation

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Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 1999-01-13
    Type: Initial release
  • Version 1.1: 2008-03-24
    Changes: Version format compliance
  • Version 1.2: 2011-07-13
    Changes: Version format compliance
  • Version 1.3: 2022-03-09
    Changes: Database references, Derived calculations, Structure summary
  • Version 1.4: 2024-05-29
    Changes: Data collection