The NMR ensemble structure of the Itk SH2 domain bound to a phosphopeptide
2EU0
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  •   Molecular Description Hide
    Classification: Transferase
    Structure Weight: 13355.13
    Molecule: Tyrosine-protein kinase ITK/TSK
    Polymer: 1 Type: protein Length: 109
    Chains: A
    EC#: 2.7.10.2   
    Fragment: SH2 domain
    Organism: Mus musculus
    Gene Names: Itk Emt Tlk Tsk
    UniProtKB: Protein Feature View | Search PDB | Q03526  
    Molecule: Lymphocyte cytosolic protein 2 phosphopeptide fragment
    Polymer: 2 Type: protein Length: 8
    Chains: B
    Fragment: phosphopeptide fragment, sequence database residues 143-148
    Organism: Mus musculus
    Gene Name: Lcp2
    UniProtKB: Protein Feature View | Search PDB | Q60787  
     

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  •   Source Hide
    Polymer: 1
    Scientific Name: Mus musculus   Taxonomy   Common Name: House mouse Expression System: Escherichia coli bl21(de3)  
    Polymer: 2
    Scientific Name: Synthetic construct (Mus musculus)   Taxonomy    
     
  •   Related PDB Entries Hide
    Identifier Details
    1LUI   NMR Structure of the Itk SH2 domain, Pro287cis, 20 low energy structures 
    1LUK   NMR Structure of the Itk SH2 domain, Pro287cis, energy minimized average structure 
    1LUM   NMR Structure of the Itk SH2 domain, Pro287trans, 20 low energy structures 
    1LUN   NMR Structure of the Itk SH2 domain, Pro287trans, energy minimized average structure 
    2ETZ   The NMR minimized average structure of the Itk SH2 domain bound to a phosphopeptide 
     
  •   Modified Residues Hide
    Identifier Formula Parent Type
    ACE
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    ACE C2 H4 O nonPolymer
    PTR
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    PTR C9 H12 N O6 P TYR lPeptideLinking
    NH2
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    NH2 H2 N nonPolymer
     
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Data in orange boxes are gathered from external resources (when available).
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  •   Deposition Summary Hide
    Authors:   Sundd, M.,  Pletneva, E.V.,  Fulton, D.B.,  Andreotti, A.H.

    Deposition:   2005-10-27
    Release:   2006-02-07
    Last Modified (REVDAT):   2009-02-24
     
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    2011-07-13
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  •   Experimental Details Hide
    Method:   SOLUTION NMR
    Exp. Data:
    N/A
    NMR Ensemble
    Conformers Calculated:     200
    Conformers Submitted:     20
    Selection Criteria: structures with the least restraint violations
    NMR Refine
    Method: SOLUTION NMR