Download MendeleyLigand Structures Of Biotin Protein Ligase From Pyrococcus Horikoshii OT3
Bagautdinov, B., Kunishima, N.To be published.
2DJZ
Crystal Structure Of Biotin Protein Ligase From Pyrococcus Horikoshii OT3 in complex with Biotinyl-5'-AMP, K111A mutation
- PDB DOI: https://doi.org/10.2210/pdb2DJZ/pdb
- Classification: LIGASE
- Organism(s): Pyrococcus horikoshii OT3
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2006-04-06 Released: 2006-10-06
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.85 Å
- R-Value Free: 0.200
- R-Value Work: 0.181
- R-Value Observed: 0.181
This is version 1.4 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| 235aa long hypothetical biotin-[acetyl-CoA-carboxylase] ligase | 235 | Pyrococcus horikoshii OT3 | Mutation(s): 1 Gene Names: birA EC: 6.3.4.15 |  | |
UniProt | |||||
Find proteins for O57883 (Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)) Explore O57883 Go to UniProtKB: O57883 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | O57883 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| BT5 Query on BT5 | C [auth A], D [auth A] | BIOTINYL-5-AMP C20 H28 N7 O9 P S UTQCSTJVMLODHM-RHCAYAJFSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.85 Å
- R-Value Free: 0.200
- R-Value Work: 0.181
- R-Value Observed: 0.181
- Space Group: P 1 21 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 38.194 | α = 90 |
| b = 82.787 | β = 101.52 |
| c = 72.324 | γ = 90 |
| Software Name | Purpose |
|---|---|
| HKL-2000 | data collection |
| SCALEPACK | data scaling |
| CNS | refinement |
| HKL-2000 | data reduction |
| CNS | phasing |
Entry History
Deposition Data
- Released Date: 2006-10-06 Deposition Author(s): Bagautdinov, B., Kunishima, N., RIKEN Structural Genomics/Proteomics Initiative (RSGI)
Revision History (Full details and data files)
- Version 1.0: 2006-10-06
Type: Initial release - Version 1.1: 2008-04-30
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Source and taxonomy, Version format compliance - Version 1.3: 2021-11-10
Changes: Database references, Derived calculations - Version 1.4: 2023-10-25
Changes: Data collection, Refinement description
