Download MendeleyThe Structures of Aspartate Aminotransferase with Mutations of Non-Active-Site Residues
Tanaka, Y., Nakagawa, N., Tada, H., Yano, T., Masui, R., Kuramitsu, S.To be published.
2D5Y
Aspartate Aminotransferase Mutant MC With Isovaleric Acid
- PDB DOI: https://doi.org/10.2210/pdb2D5Y/pdb
- Classification: TRANSFERASE
- Organism(s): Escherichia coli
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2005-11-08 Released: 2006-11-14
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.98 Å
- R-Value Free: 0.216
- R-Value Work: 0.184
- R-Value Observed: 0.184
This is version 1.3 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Aspartate aminotransferase | 396 | Escherichia coli | Mutation(s): 8 Gene Names: aspC EC: 2.6.1.1 |  | |
UniProt | |||||
Find proteins for P00509 (Escherichia coli (strain K12)) Explore P00509 Go to UniProtKB: P00509 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P00509 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| PLP Query on PLP | B [auth A] | PYRIDOXAL-5'-PHOSPHATE C8 H10 N O6 P NGVDGCNFYWLIFO-UHFFFAOYSA-N |  | ||
| IVA Query on IVA | C [auth A] | ISOVALERIC ACID C5 H10 O2 GWYFCOCPABKNJV-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.98 Å
- R-Value Free: 0.216
- R-Value Work: 0.184
- R-Value Observed: 0.184
- Space Group: C 2 2 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 157.234 | α = 90 |
| b = 85.152 | β = 90 |
| c = 78.731 | γ = 90 |
| Software Name | Purpose |
|---|---|
| CNS | refinement |
| DENZO | data reduction |
| SCALEPACK | data scaling |
Entry History
Deposition Data
- Released Date: 2006-11-14 Deposition Author(s): Tanaka, Y., Nakagawa, N., Tada, H., Yano, T., Masui, R., Kuramitsu, S.
Revision History (Full details and data files)
- Version 1.0: 2006-11-14
Type: Initial release - Version 1.1: 2008-04-30
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Derived calculations, Version format compliance - Version 1.3: 2021-11-10
Changes: Database references, Derived calculations
