Download MendeleyStructural Properties of Wild-Type and Two Artt Motif Mutants Clostridium Botulinum C3 Exoenzyme Isoform 1 in Different Substrate Complexed States and Crystal Forms.
Stura, E.A., Menetrey, J., Flatau, G., Boquet, P., Menez, A.To be published.
2C8G
Structure of the PN loop Q182A mutant C3bot1 Exoenzyme (Free state, crystal form I)
- PDB DOI: https://doi.org/10.2210/pdb2C8G/pdb
- Classification: TRANSFERASE
- Organism(s): Clostridium botulinum
- Expression System: Escherichia coli BL21
- Mutation(s): Yes
- Deposited: 2005-12-03 Released: 2007-02-27
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 Å
- R-Value Free: 0.247
- R-Value Work: 0.212
- R-Value Observed: 0.213
wwPDB Validation 3D Report Full Report
This is version 1.3 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| MONO-ADP-RIBOSYLTRANSFERASE C3 | 211 | Clostridium botulinum | Mutation(s): 1 EC: 2.4.2 |  | |
UniProt | |||||
Find proteins for P15879 (Clostridium botulinum D phage) Explore P15879 Go to UniProtKB: P15879 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P15879 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| SO4 Query on SO4 | E [auth B], F [auth C], G [auth D] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 Å
- R-Value Free: 0.247
- R-Value Work: 0.212
- R-Value Observed: 0.213
- Space Group: C 1 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 105.671 | α = 90 |
| b = 74.951 | β = 102.46 |
| c = 121.199 | γ = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| DENZO | data reduction |
| SCALEPACK | data scaling |
| AMoRE | phasing |
Entry History
Deposition Data
- Released Date: 2007-02-27 Deposition Author(s): Stura, E.A., Menetrey, J., Flatau, G., Boquet, P., Menez, A.
Revision History (Full details and data files)
- Version 1.0: 2007-02-27
Type: Initial release - Version 1.1: 2011-05-08
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Version format compliance - Version 1.3: 2023-12-13
Changes: Data collection, Database references, Other, Refinement description
