2BZD
Galactose recognition by the carbohydrate-binding module of a bacterial sialidase.
- PDB DOI: https://doi.org/10.2210/pdb2BZD/pdb
- Entry: 2BZD supersedes: 2BQ9
- Classification: HYDROLASE
- Organism(s): Micromonospora viridifaciens
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Deposited: 2005-08-16 Released: 2005-08-19
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 Å
- R-Value Free: 0.267
- R-Value Work: 0.193
- R-Value Observed: 0.197
This is version 1.3 of the entry. See complete history.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| BACTERIAL SIALIDASE | 601 | Micromonospora viridifaciens | Mutation(s): 1 EC: 3.2.1.18 |  | |
UniProt | |||||
Find proteins for Q02834 (Micromonospora viridifaciens) Explore Q02834 Go to UniProtKB: Q02834 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q02834 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| GAL Query on GAL | E [auth A], H [auth B], K [auth C] | beta-D-galactopyranose C6 H12 O6 WQZGKKKJIJFFOK-FPRJBGLDSA-N |  | ||
| GOL Query on GOL | F [auth A], I [auth B], L [auth C] | GLYCEROL C3 H8 O3 PEDCQBHIVMGVHV-UHFFFAOYSA-N |  | ||
| NA Query on NA | D [auth A], G [auth B], J [auth C] | SODIUM ION Na FKNQFGJONOIPTF-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 Å
- R-Value Free: 0.267
- R-Value Work: 0.193
- R-Value Observed: 0.197
- Space Group: P 32 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 141.457 | α = 90 |
| b = 141.457 | β = 90 |
| c = 158.88 | γ = 120 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| MOSFLM | data reduction |
| SCALA | data scaling |
| AMoRE | phasing |
Entry History
Deposition Data
- Released Date: 2005-08-19 Deposition Author(s): Newstead, S.L., Taylor, G.
- This entry supersedes: 2BQ9
Revision History (Full details and data files)
- Version 1.0: 2005-08-19
Type: Initial release - Version 1.1: 2011-07-13
Changes: Advisory, Version format compliance - Version 1.2: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Data collection, Derived calculations, Other, Structure summary - Version 1.3: 2023-12-13
Changes: Data collection, Database references, Refinement description, Structure summary
