Download MendeleyCrystal structure of Type I restriction enzyme EcoKI M protein (EC 2.1.1.72) (M.EcoKI).
Rajashankar, K.R., Kniewel, R., Lima, C.D.To be published.
2AR0
Crystal structure of Type I restriction enzyme EcoKI M protein (EC 2.1.1.72) (M.EcoKI)
- PDB DOI: https://doi.org/10.2210/pdb2AR0/pdb
- Classification: TRANSCRIPTION,TRANSFERASE
- Organism(s): Escherichia coli
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2005-08-18 Released: 2005-08-30
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.80 Å
- R-Value Free: 0.261
- R-Value Work: 0.224
- R-Value Observed: 0.224
wwPDB Validation 3D Report Full Report
This is version 1.4 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Type I restriction enzyme EcoKI M protein | 541 | Escherichia coli | Mutation(s): 10 Gene Names: hsdM, hsm EC: 2.1.1.72 |  | |
UniProt | |||||
Find proteins for P08957 (Escherichia coli (strain K12)) Explore P08957 Go to UniProtKB: P08957 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P08957 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| UNX Query on UNX | C [auth A] D [auth A] E [auth A] F [auth A] G [auth A] | UNKNOWN ATOM OR ION X |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| MSE Query on MSE | A, B | L-PEPTIDE LINKING | C5 H11 N O2 Se |  | MET |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.80 Å
- R-Value Free: 0.261
- R-Value Work: 0.224
- R-Value Observed: 0.224
- Space Group: P 41
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 105.427 | α = 90 |
| b = 105.427 | β = 90 |
| c = 138.279 | γ = 90 |
| Software Name | Purpose |
|---|---|
| DENZO | data reduction |
| SCALEPACK | data scaling |
| SOLVE | phasing |
| CNS | refinement |
Entry History
Deposition Data
- Released Date: 2005-08-30 Deposition Author(s): Rajashankar, K.R., Kniewel, R., Lima, C.D., Burley, S.K., New York SGX Research Center for Structural Genomics (NYSGXRC)
Revision History (Full details and data files)
- Version 1.0: 2005-08-30
Type: Initial release - Version 1.1: 2008-04-30
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Version format compliance - Version 1.3: 2021-02-03
Changes: Database references, Derived calculations, Structure summary - Version 1.4: 2024-04-03
Changes: Data collection, Database references, Refinement description
