2A0M

Structural genomics of pathogenic protozoa: an overview.

Fan, E., Baker, D., Fields, S., Gelb, M.H., Buckner, F.S., Van Voorhis, W.C., Phizicky, E., Dumont, M., Mehlin, C., Grayhack, E., Sullivan, M., Verlinde, C., Detitta, G., Meldrum, D.R., Merritt, E.A., Earnest, T., Soltis, M., Zucker, F., Myler, P.J., Schoenfeld, L., Kim, D., Worthey, L., Lacount, D., Vignali, M., Li, J., Mondal, S., Massey, A., Carroll, B., Gulde, S., Luft, J., Desoto, L., Holl, M., Caruthers, J., Bosch, J., Robien, M., Arakaki, T., Holmes, M., Le Trong, I., Hol, W.G.

(2008) Methods Mol Biol 426: 497-513

• PubMed: 18542886 Search on PubMed
• DOI: https://doi.org/10.1007/978-1-60327-058-8_33
• Primary Citation of Related Structures:  
  2A0M


• PubMed Abstract: 
  

  The Structural Genomics of Pathogenic Protozoa (SGPP) Consortium aimed to determine crystal structures of proteins from trypanosomatid and malaria parasites in a high throughput manner. The pipeline of target selection, protein production, crystallization, and structure determination, is sketched. Special emphasis is given to a number of technology developments including domain prediction, the use of "co-crystallants," and capillary crystallization. "Fragment cocktail crystallography" for medical structural genomics is also described.

   View More

---

Organizational Affiliation: 

Department of Biochemistry, University of Washington, Seattle, WA, USA.