2Y6S

Structure of an Ebolavirus-protective antibody in complex with its mucin-domain linear epitope


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.80 Å
  • R-Value Free: 0.287 
  • R-Value Work: 0.236 
  • R-Value Observed: 0.239 

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This is version 1.2 of the entry. See complete history


Literature

Structure of an Ebola Virus-Protective Antibody in Complex with its Mucin-Domain Linear Epitope.

Olal, D.O.Kuehne, A.Bale, S.Halfmann, P.Hashiguchi, T.Fusco, M.L.Lee, J.E.King, L.B.Kawaoka, Y.Dye, J.M.Saphire, E.O.

(2012) J Virol 86: 2809

  • DOI: https://doi.org/10.1128/JVI.05549-11
  • Primary Citation of Related Structures:  
    2Y6S

  • PubMed Abstract: 

    Antibody 14G7 is protective against lethal Ebola virus challenge and recognizes a distinct linear epitope in the prominent mucin-like domain of the Ebola virus glycoprotein GP. The structure of 14G7 in complex with its linear peptide epitope has now been determined to 2.8 Å. The structure shows that this GP sequence forms a tandem β-hairpin structure that binds deeply into a cleft in the antibody-combining site. A key threonine at the apex of one turn is critical for antibody interaction and is conserved among all Ebola viruses. This work provides further insight into the mechanism of protection by antibodies that target the protruding, highly accessible mucin-like domain of Ebola virus and the structural framework for understanding and characterizing candidate immunotherapeutics.


  • Organizational Affiliation

    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California, USA.


Macromolecules
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Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
LIGHT CHAINA [auth C],
D [auth L]
217Mus musculusMutation(s): 0 
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  • Reference Sequence
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Entity ID: 2
MoleculeChains Sequence LengthOrganismDetailsImage
HEAVY CHAINB [auth D],
C [auth H]
213Mus musculusMutation(s): 0 
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  • Reference Sequence

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Entity ID: 3
MoleculeChains Sequence LengthOrganismDetailsImage
ENVELOPE GLYCOPROTEINE [auth P],
F [auth Q]
17Ebola virus - Zaire (1995)Mutation(s): 0 
UniProt
Find proteins for P87666 (Zaire ebolavirus (strain Kikwit-95))
Explore P87666 
Go to UniProtKB:  P87666
Entity Groups  
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UniProt GroupP87666
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.80 Å
  • R-Value Free: 0.287 
  • R-Value Work: 0.236 
  • R-Value Observed: 0.239 
  • Space Group: P 1 2 1
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 89.554α = 90
b = 68.376β = 112.5
c = 92.827γ = 90
Software Package:
Software NamePurpose
PHENIXrefinement
HKL-2000data reduction
HKL-2000data scaling
PHENIXphasing

Structure Validation

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Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2012-01-11
    Type: Initial release
  • Version 1.1: 2012-02-22
    Changes: Other
  • Version 1.2: 2023-12-20
    Changes: Data collection, Database references, Other, Refinement description