2RV8

Solution structure of the PhoP DNA-binding domain from Mycobacterium tuberculosis.

(2015) J Biomol NMR 63: 111-117

• PubMed: 26209027 Search on PubMedSearch on PubMed Central
• DOI: https://doi.org/10.1007/s10858-015-9965-0
• Primary Citation of Related Structures:  
  2RV8


• PubMed Abstract: 
  

  Tuberculosis caused by Mycobacterium tuberculosis is a leading cause of death world-wide. The PhoP protein is required for virulence and is part of the PhoPR two-component system that regulates gene expression. The NMR-derived solution structure of the PhoP C-terminal DNA-binding domain is reported. Residues 150 to 246 form a structured domain that contains a winged helix-turn-helix motif. We provide evidence that the transactivation loop postulated to contact RNA polymerase is partially disordered in solution, and that the polypeptide that connects the DNA-binding domain to the regulatory domain is unstructured.

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Organizational Affiliation: 

Department of Chemistry and Biochemistry, University of California, Los Angeles, 602 Boyer Hall, Los Angeles, CA, 90095, USA.