2PZ9

In situ proteolysis for protein crystallization and structure determination.

Dong, A., Xu, X., Edwards, A.M., Chang, C., Chruszcz, M., Cuff, M., Cymborowski, M., Di Leo, R., Egorova, O., Evdokimova, E., Filippova, E., Gu, J., Guthrie, J., Ignatchenko, A., Joachimiak, A., Klostermann, N., Kim, Y., Korniyenko, Y., Minor, W., Que, Q., Savchenko, A., Skarina, T., Tan, K., Yakunin, A., Yee, A., Yim, V., Zhang, R., Zheng, H., Akutsu, M., Arrowsmith, C., Avvakumov, G.V., Bochkarev, A., Dahlgren, L.G., Dhe-Paganon, S., Dimov, S., Dombrovski, L., Finerty, P., Flodin, S., Flores, A., Graslund, S., Hammerstrom, M., Herman, M.D., Hong, B.S., Hui, R., Johansson, I., Liu, Y., Nilsson, M., Nedyalkova, L., Nordlund, P., Nyman, T., Min, J., Ouyang, H., Park, H.W., Qi, C., Rabeh, W., Shen, L., Shen, Y., Sukumard, D., Tempel, W., Tong, Y., Tresagues, L., Vedadi, M., Walker, J.R., Weigelt, J., Welin, M., Wu, H., Xiao, T., Zeng, H., Zhu, H.

(2007) Nat Methods 4: 1019-1021

• PubMed: 17982461 Search on PubMedSearch on PubMed Central
• DOI: https://doi.org/10.1038/nmeth1118
• Primary Citation of Related Structures:  
  2PZ9


• PubMed Abstract: 
  

  We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.

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Organizational Affiliation: 

Structural Genomics Consortium, University of Toronto, 100 College Street, Toronto, Ontario M5G 1L5, Canada.