2PYS
Crystal Structure of a Five Site Mutated Cyanovirin-N with a Mannose Dimer Bound at 1.8 A Resolution
- PDB DOI: https://doi.org/10.2210/pdb2PYS/pdb
- Classification: SUGAR BINDING PROTEIN
- Organism(s): Nostoc ellipsosporum
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2007-05-16 Released: 2007-07-31
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.80 Å
- R-Value Free: 0.215
- R-Value Work: 0.169
- R-Value Observed: 0.171
wwPDB Validation 3D Report Full Report
This is version 2.2 of the entry. See complete history.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Cyanovirin-N | 109 | Nostoc ellipsosporum | Mutation(s): 5 Gene Names: CYANOVIRN-N |  | |
UniProt | |||||
Find proteins for P81180 (Nostoc ellipsosporum) Explore P81180 Go to UniProtKB: P81180 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P81180 | ||||
Sequence AnnotationsExpand | |||||
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Oligosaccharides
Biologically Interesting Molecules (External Reference) 1 Unique
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| ID | Chains | Name | Type/Class | 2D Diagram | 3D Interactions |
| PRD_900111 Query on PRD_900111 | C, D | 2alpha-alpha-mannobiose | Oligosaccharide / Metabolism |  | |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.80 Å
- R-Value Free: 0.215
- R-Value Work: 0.169
- R-Value Observed: 0.171
- Space Group: P 1 21 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 49.226 | α = 90 |
| b = 38.281 | β = 99.69 |
| c = 55.794 | γ = 90 |
| Software Name | Purpose |
|---|---|
| PHASER | phasing |
| REFMAC | refinement |
| CrystalClear | data collection |
| HKL-2000 | data reduction |
| HKL-2000 | data scaling |
Entry History
Deposition Data
- Released Date: 2007-07-31 Deposition Author(s): Fromme, R., Katilene, Z., Fromme, P., Ghirlanda, G.
Revision History (Full details and data files)
- Version 1.0: 2007-07-31
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance - Version 2.0: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Advisory, Atomic model, Data collection, Database references, Derived calculations, Structure summary - Version 2.1: 2021-10-20
Changes: Database references, Structure summary - Version 2.2: 2023-08-30
Changes: Data collection, Refinement description
