2P3J
Crystal structure of the Arg101Ala mutant protein of Rhesus rotavirus VP8*
- PDB DOI: https://doi.org/10.2210/pdb2P3J/pdb
- Classification: VIRAL PROTEIN
- Organism(s): Simian rotavirus A strain RRV
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Deposited: 2007-03-09 Released: 2008-03-11
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.90 Å
- R-Value Free: 0.206
- R-Value Work: 0.161
- R-Value Observed: 0.163
This is version 1.5 of the entry. See complete history.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| VP4 | 161 | Simian rotavirus A strain RRV | Mutation(s): 1 |  | |
UniProt | |||||
Find proteins for P12473 (Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3])) Explore P12473 Go to UniProtKB: P12473 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P12473 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| MNA Query on MNA | C [auth A] | 2-O-methyl-5-N-acetyl-alpha-D-neuraminic acid C12 H21 N O9 NJRVVFURCKKXOD-MIDKXNQYSA-N |  | ||
| SO4 Query on SO4 | B [auth A] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.90 Å
- R-Value Free: 0.206
- R-Value Work: 0.161
- R-Value Observed: 0.163
- Space Group: P 41 21 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 48.531 | α = 90 |
| b = 48.531 | β = 90 |
| c = 131.403 | γ = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| PROTEUM PLUS | data collection |
| SAINT | data reduction |
| LSCALE | data scaling |
| AMoRE | phasing |
Entry History
Deposition Data
- Released Date: 2008-03-11 Deposition Author(s): Blanchard, H.
Revision History (Full details and data files)
- Version 1.0: 2008-03-11
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance - Version 1.2: 2017-10-18
Changes: Refinement description - Version 1.3: 2019-10-23
Changes: Data collection, Database references - Version 1.4: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Data collection, Derived calculations, Structure summary - Version 1.5: 2023-10-25
Changes: Data collection, Database references, Refinement description, Structure summary
