2MP8

NMR structure of NKR-5-3B


Experimental Data Snapshot

  • Method: SOLUTION NMR
  • Conformers Calculated: 50 
  • Conformers Submitted: 20 
  • Selection Criteria: structures with the lowest energy 

wwPDB Validation   3D Report Full Report


This is version 1.1 of the entry. See complete history


Literature

Identification, Characterization, and Three-Dimensional Structure of the Novel Circular Bacteriocin, Enterocin NKR-5-3B, from Enterococcus faecium

Himeno, K.Rosengren, K.J.Inoue, T.Perez, R.H.Colgrave, M.L.Lee, H.S.Chan, L.Y.Henriques, S.T.Fujita, K.Ishibashi, N.Zendo, T.Wilaipun, P.Nakayama, J.Leelawatcharamas, V.Jikuya, H.Craik, D.J.Sonomoto, K.

(2015) Biochemistry 54: 4863-4876

  • DOI: https://doi.org/10.1021/acs.biochem.5b00196
  • Primary Citation of Related Structures:  
    2MP8

  • PubMed Abstract: 

    Enterocin NKR-5-3B, one of the multiple bacteriocins produced by Enterococcus faecium NKR-5-3, is a 64-amino acid novel circular bacteriocin that displays broad-spectrum antimicrobial activity. Here we report the identification, characterization, and three-dimensional nuclear magnetic resonance solution structure determination of enterocin NKR-5-3B. Enterocin NKR-5-3B is characterized by four helical segments that enclose a compact hydrophobic core, which together with its circular backbone impart high stability and structural integrity. We also report the corresponding structural gene, enkB, that encodes an 87-amino acid precursor peptide that undergoes a yet to be described enzymatic processing that involves adjacent cleavage and ligation of Leu(24) and Trp(87) to yield the mature (circular) enterocin NKR-5-3B.


  • Organizational Affiliation

    †Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
NKR-5-3B64Enterococcus faecalisMutation(s): 0 
Membrane Entity: Yes 
UniProt
Find proteins for A0A0M3KKS4 (Enterococcus faecalis)
Explore A0A0M3KKS4 
Go to UniProtKB:  A0A0M3KKS4
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupA0A0M3KKS4
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: SOLUTION NMR
  • Conformers Calculated: 50 
  • Conformers Submitted: 20 
  • Selection Criteria: structures with the lowest energy 

Structure Validation

View Full Validation Report



Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2015-05-13
    Type: Initial release
  • Version 1.1: 2016-06-01
    Changes: Database references