2LF6

Solution NMR structure of HopABPph1448_220_320 from Pseudomonas syringae pv. phaseolicola str. 1448A, Midwest Center for Structural Genomics target APC40132.4 and Northeast Structural Genomics Consortium target PsT3A


Experimental Data Snapshot

  • Method: SOLUTION NMR
  • Conformers Calculated: 100 
  • Conformers Submitted: 20 
  • Selection Criteria: structures with the lowest energy 

wwPDB Validation   3D Report Full Report


This is version 1.4 of the entry. See complete history


Literature

Structural Analysis of HopPmaL Reveals the Presence of a Second Adaptor Domain Common to the HopAB Family of Pseudomonas syringae Type III Effectors.

Singer, A.U.Wu, B.Yee, A.Houliston, S.Xu, X.Cui, H.Skarina, T.Garcia, M.Semesi, A.Arrowsmith, C.H.Savchenko, A.

(2012) Biochemistry 51: 1-3

  • DOI: https://doi.org/10.1021/bi2013883
  • Primary Citation of Related Structures:  
    2LF3, 2LF6, 3SVI, 3TJY

  • PubMed Abstract: 

    HopPmaL is a member of the HopAB family of type III effectors present in the phytopathogen Pseudomonas syringae. Using both X-ray crystallography and solution nuclear magnetic resonance, we demonstrate that HopPmaL contains two structurally homologous yet functionally distinct domains. The N-terminal domain corresponds to the previously described Pto-binding domain, while the previously uncharacterised C-terminal domain spans residues 308-385. While structurally similar, these domains do not share significant sequence similarity and most importantly demonstrate significant differences in key residues involved in host protein recognition, suggesting that each of them targets a different host protein.


  • Organizational Affiliation

    Department of Chemical Engineering and Applied Chemistry, Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario M5G 1L6, Canada.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Effector protein hopAB1101Pseudomonas savastanoi pv. phaseolicola 1448AMutation(s): 0 
Gene Names: hopAB1virPphAPSPPH_A0127
UniProt
Find proteins for Q48B61 (Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6))
Explore Q48B61 
Go to UniProtKB:  Q48B61
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupQ48B61
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: SOLUTION NMR
  • Conformers Calculated: 100 
  • Conformers Submitted: 20 
  • Selection Criteria: structures with the lowest energy 

Structure Validation

View Full Validation Report



Entry History 

Revision History  (Full details and data files)

  • Version 1.0: 2011-07-13
    Type: Initial release
  • Version 1.1: 2012-01-11
    Changes: Database references
  • Version 1.2: 2012-01-25
    Changes: Database references
  • Version 1.3: 2012-02-22
    Changes: Structure summary
  • Version 1.4: 2023-06-14
    Changes: Data collection, Database references, Other