2KVE

C-terminal domain of mesencephalic astrocyte-derived neurotrophic factor (MANF)

PDB DOI: https://doi.org/10.2210/pdb2KVE/pdb
BMRB: 16776

Classification: HORMONE
Organism(s): Homo sapiens
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2010-03-12 Released: 2010-11-03
Deposition Author(s): Hellman, M.H., Saarma, M., Permi, P.

Experimental Data Snapshot

Method: SOLUTION NMR
Conformers Calculated: 400
Conformers Submitted: 15
Selection Criteria: structures with the least restraint violations

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

Mesencephalic Astrocyte-derived Neurotrophic Factor (MANF) Has a Unique Mechanism to Rescue Apoptotic Neurons

Hellman, M.H., Arumae, U., Yu, L.-Y., Lindholm, P., Peranen, J., Saarma, M., Permi, P.

(2011) J Biol Chem 286: 2675-2680

PubMed: 21047780 Search on PubMedSearch on PubMed Central
DOI: https://doi.org/10.1074/jbc.M110.146738
Primary Citation of Related Structures:
2KVD, 2KVE

PubMed Abstract:
Mesencephalic astrocyte-derived neurotrophic factor (MANF) protects neurons and repairs the Parkinson disease-like symptoms in a rat 6-hydroxydopamine model. We show a three-dimensional solution structure of human MANF that differs drastically from other neurotrophic factors. Remarkably, the C-terminal domain of MANF (C-MANF) is homologous to the SAP domain of Ku70, a well known inhibitor of proapoptotic Bax (Bcl-2-associated X protein). Cellular studies confirm that MANF and C-MANF protect neurons intracellularly as efficiently as Ku70.

Organizational Affiliation

Program in Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, FI-00014 Helsinki, Finland.

Macromolecule Content

Total Structure Weight: 7.48 kDa
Atom Count: 520
Modelled Residue Count: 65
Deposited Residue Count: 65
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Mesencephalic astrocyte-derived neurotrophic factor	A	65	Homo sapiens	Mutation(s): 0
UniProt & NIH Common Fund Data Resources
Find proteins for P55145 (Homo sapiens) Explore P55145 Go to UniProtKB: P55145
PHAROS: P55145 GTEx: ENSG00000145050
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P55145
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: SOLUTION NMR
Conformers Calculated: 400
Conformers Submitted: 15
Selection Criteria: structures with the least restraint violations

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2010-11-03

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2010-11-03
Type: Initial release
Version 1.1: 2011-07-13
Changes: Version format compliance

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.