2JHP

The structure of bluetongue virus VP4 reveals a multifunctional RNA- capping production-line


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.50 Å
  • R-Value Free: 0.289 
  • R-Value Work: 0.226 
  • R-Value Observed: 0.229 

wwPDB Validation   3D Report Full Report


Ligand Structure Quality Assessment 


This is version 1.3 of the entry. See complete history


Literature

Bluetongue Virus Vp4 is an RNA-Capping Assembly Line.

Sutton, G.Grimes, J.M.Stuart, D.I.Roy, P.

(2007) Nat Struct Mol Biol 14: 449

  • DOI: https://doi.org/10.1038/nsmb1225
  • Primary Citation of Related Structures:  
    2JH8, 2JH9, 2JHA, 2JHC, 2JHP

  • PubMed Abstract: 

    Eukaryotic organisms cap the 5' ends of their messenger RNAs by a series of four chemical reactions. Some viruses achieve this using a single molecule; the crystal structure of such an enzyme from bluetongue virus reveals an elongated modular architecture that provides a scaffold for an assemblage of active sites, two contributed by a domain of novel structure.


  • Organizational Affiliation

    Division of Structural Biology, The Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Headington, Oxford, OX3 7BN, UK.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
VP4 CORE PROTEIN644Bluetongue virus (serotype 10 / American isolate)Mutation(s): 0 
UniProt
Find proteins for P07132 (Bluetongue virus 10 (isolate USA))
Explore P07132 
Go to UniProtKB:  P07132
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP07132
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.50 Å
  • R-Value Free: 0.289 
  • R-Value Work: 0.226 
  • R-Value Observed: 0.229 
  • Space Group: P 65 2 2
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 76.242α = 90
b = 76.242β = 90
c = 421.508γ = 120
Software Package:
Software NamePurpose
piratemodel building
SCALEPACKdata scaling
SHELXCDphasing
SHELXDphasing
SHARPphasing
piratephasing
REFMACrefinement

Structure Validation

View Full Validation Report



Ligand Structure Quality Assessment 


Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2007-04-10
    Type: Initial release
  • Version 1.1: 2011-07-13
    Changes: Refinement description, Version format compliance
  • Version 1.2: 2017-06-21
    Changes: Refinement description, Source and taxonomy
  • Version 1.3: 2019-05-08
    Changes: Data collection, Experimental preparation