2JH8

The structure of bluetongue virus VP4 reveals a multifunctional RNA- capping production-line


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 3.22 Å
  • R-Value Free: 0.341 
  • R-Value Work: 0.246 
  • R-Value Observed: 0.246 

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Ligand Structure Quality Assessment 


This is version 2.0 of the entry. See complete history


Literature

Bluetongue Virus Vp4 is an RNA-Capping Assembly Line.

Sutton, G.Grimes, J.M.Stuart, D.I.Roy, P.

(2007) Nat Struct Mol Biol 14: 449

  • DOI: https://doi.org/10.1038/nsmb1225
  • Primary Citation of Related Structures:  
    2JH8, 2JH9, 2JHA, 2JHC, 2JHP

  • PubMed Abstract: 

    Eukaryotic organisms cap the 5' ends of their messenger RNAs by a series of four chemical reactions. Some viruses achieve this using a single molecule; the crystal structure of such an enzyme from bluetongue virus reveals an elongated modular architecture that provides a scaffold for an assemblage of active sites, two contributed by a domain of novel structure.


  • Organizational Affiliation

    Division of Structural Biology, The Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Headington, Oxford, OX3 7BN, UK.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
VP4 CORE PROTEIN644Bluetongue virus (serotype 10 / American isolate)Mutation(s): 0 
UniProt
Find proteins for Q65751 (Bluetongue virus 10)
Explore Q65751 
Go to UniProtKB:  Q65751
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupQ65751
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 3.22 Å
  • R-Value Free: 0.341 
  • R-Value Work: 0.246 
  • R-Value Observed: 0.246 
  • Space Group: P 65 2 2
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 75.055α = 90
b = 75.055β = 90
c = 419.834γ = 120
Software Package:
Software NamePurpose
CNSrefinement
DENZOdata reduction
SCALEPACKdata scaling

Structure Validation

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Ligand Structure Quality Assessment 


Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2007-04-10
    Type: Initial release
  • Version 1.1: 2011-05-08
    Changes: Version format compliance
  • Version 1.2: 2011-07-13
    Changes: Version format compliance
  • Version 1.3: 2019-10-16
    Changes: Data collection, Other, Source and taxonomy
  • Version 2.0: 2024-03-06
    Changes: Data collection, Database references, Non-polymer description, Structure summary