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Structure of ribosomal protein L1-mRNA complex at 2.1 resolution.
 
 
DOI:10.2210/pdb2hw8/pdb   NDB ID: PR0225
2HW8
 
Primary Citation
spinning wheel
 
 
  •  
    Move Section Molecular Description Hide
    Classification: Structural Protein/rna
    Structure Weight: 36684.03
     
    Molecule:36-MER
    Polymer:1Type:polyribonucleotideLength:36
    Chains:B
    Molecule:50S ribosomal protein L1
    Polymer:2Type:polypeptide(L)Length:228
    Chains:A
     
     
  •  
    Move Section Source Hide
    Polymer: 1
    Scientific Name: Synthetic construct Go to NCBI Taxonomy entry  
    Polymer: 2
    Scientific Name: Thermus thermophilus Go to NCBI Taxonomy entry Expression System: Escherichia coli bl21(de3)  
     
     
  •  
    Move Section Related PDB Entries Hide
    Id Details
    1U63  The crystal structure of ribosomal protein L1 from Methanococcus jannaschii in complex with a specific fragment of its mRNA. 
    1ZHO  The crystal structure at 2.6 A of ribosomal protein L1 from Thermus thermophilus in complex with a 38 nt fragment of L1 mRNA from Methanoccocus vannielii. Space group is P21. 
     
     
  •  
    Move Section Ligand Chemical Component Hide
    Identifier Name Formula Interaction View Links
    BU1     1,4-BUTANEDIOL C4 H10 O2 3DLigand Explorer Link out to Ligand Expos:BU1 Link out to SuperLigands:BU1 Link out to SuperHapten:BU1
    K     POTASSIUM ION K 3DLigand Explorer Link out to Ligand Expos:K Link out to SuperLigands:K Link out to SuperHapten:K
    MG     MAGNESIUM ION Mg 3DLigand Explorer Link out to Ligand Expos:MG Link out to SuperLigands:MG Link out to SuperHapten:MG
     
     
  •  
    Move Section Modified Residues Hide
    Identifier Name Formula Interaction View Links
    MSE SELENOMETHIONINE (parent: MET)(type: lPeptideLinking) C5 H11 N O2 Se 3D Ligand Explorer Link out to Ligand Expos:MSE Link out to SuperLigands:MSE Link out to SuperHapten:MSE 2D
     
     
  •  
    Move Section Derived Data Hide
     
     
 
 
  •  
    Move Section Deposition Summary Hide
    Authors:   Tishchenko, S.,   Nikonova, E.,   Nikulin, A.,   Nevskaya, N.,   Volchkov, S.,   Piendl, W.,   Garber, M.,   Nikonov, S.

    Deposition:   2006-08-01
    Release:   2006-12-19
    Last Modified (REVDAT):   2009-02-24
     
     
  •  
    Move Section Experimental Details Hide
    Method:   X-RAY DIFFRACTION
    Experimental Data:   Download Structure Factors  [ EDS External Link to EDS ]
    View a histogram of Resolution Resolution[Å]: 2.10
    R-Value: 0.212 (obs.)
    R-Free: 0.247
    Space Group: P 65 2 2
    Unit Cell:
      Length [Å] Angles [°]
    a = 67.87 α = 90.00 
    b = 67.87 β = 90.00 
    c = 340.47 γ = 120.00