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Crystal structure of Aquifex aeolicus RNase III (D44N) complexed with product of double-stranded RNA processing
 
 
DOI:10.2210/pdb2ez6/pdb   NDB ID: PR0185
2EZ6
 
Primary Citation
spinning wheel
 
 
  •  
    Move Section Molecular Description Hide
    Classification: Hydrolase/rna
    Structure Weight: 70302.67
     
    Molecule:28-MER
    Polymer:1Type:polyribonucleotideLength:28
    Chains:C, D
    Molecule:Ribonuclease III
    Polymer:2Type:polypeptide(L)Length:221
    Chains:A, B
    EC#:3.1.26.3 Go to IUBMB EC entry  
    Mutation:D44N
     
     
  •  
    Move Section Source Hide
    Polymer: 1
    Scientific Name: Synthetic construct Go to NCBI Taxonomy entry  
    Polymer: 2
    Scientific Name: Aquifex aeolicus Go to NCBI Taxonomy entry Expression System: Escherichia coli  
     
     
  •  
    Move Section Related PDB Entries Hide
    Id Details
    1JFZ  Crystal structure of a MN(II)-complex of RNase III endonuclease domain from Aquifex aeolicus 
    1O0W  The crystal structure of RNase III from Thermotoga maritima 
    1RC5  The crystal structure of MG(II)-complex of RNase III endonuclease domain from Aquifex aeolicus 
    1RC7  Crystal structure of RNase III mutant E110K from Aquifex aeolicus complexed with ds-RNA (5'-GGCGCGCGCC-3') 
    1YYK  Crystal structure of RNase III (E110K) from Aquifex aeolicus complexed with ds-RNA (5'-CGCGAAUUCGCG-3') at 2.9-Angstrom Resolution 
    1YYO  Crystal structure of RNase III from Aquifex aeolicus complexed with ds-RNA (5'-CGCGAAUUCGCG-3') at 2.5-Angstrom Resolution 
    1YYW  Crystal structure of RNase III from Aquifex aeolicus complexed with ds-RNA (5'-AAAUAUAUAUUU-3') at 2.8-Angstrom Resolution 
    1YZ9  Crystal structure of RNase III mutant E110Q from Aquifex aeolicus complexed with ds-RNA (5'-CGAACUUCGCG-3') at 2.1-Angstrom Resolution 
     
     
  •  
    Move Section Ligand Chemical Component Hide
    Identifier Name Formula Interaction View Links
    MG     MAGNESIUM ION Mg 3DLigand Explorer Link out to Ligand Expos:MG Link out to SuperLigands:MG Link out to SuperHapten:MG
     
     
  •  
    Move Section Derived Data Hide
     
     
 
 
  •  
    Move Section Deposition Summary Hide
    Authors:   Gan, J.,   Tropea, J.E.,   Austin, B.P.,   Court, D.L.,   Waugh, D.S.,   Ji, X.

    Deposition:   2005-11-10
    Release:   2006-02-07
    Last Modified (REVDAT):   2009-02-24
     
     
  •  
    Move Section Experimental Details Hide
    Method:   X-RAY DIFFRACTION
    Experimental Data:   Download Structure Factors  [ EDS External Link to EDS ]
    View a histogram of Resolution Resolution[Å]: 2.05
    R-Value: 0.205 (obs.)
    R-Free: 0.258
    Space Group: P 21
    Unit Cell:
      Length [Å] Angles [°]
    a = 63.84 α = 90.00 
    b = 50.98 β = 104.47 
    c = 113.75 γ = 90.00