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Cocrystal structure of an RNA sulfuration enzyme MnmA and tRNA-Glu in the adenylated intermediate state
 
 
DOI:10.2210/pdb2deu/pdb   NDB ID: PR0194
2DEU
 
Primary Citation
spinning wheel
 
 
  •  
    Move Section Molecular Description Hide
    Classification: Transferase/rna
    Structure Weight: 134468.56
     
    Molecule:tRNA
    Polymer:1Type:polyribonucleotideLength:76
    Chains:C, D
    Molecule:tRNA-specific 2-thiouridylase mnmA
    Polymer:2Type:polypeptide(L)Length:380
    Chains:A, B
    EC#:2.8.1.- Go to IUBMB EC entry  
     
     
  •  
    Move Section Source Hide
    Polymer: 1
    Scientific Name: Synthetic construct Go to NCBI Taxonomy entry  
    Polymer: 2
    Scientific Name: Escherichia coli Go to NCBI Taxonomy entry Expression System: Escherichia coli  
     
     
  •  
    Move Section Related PDB Entries Hide
    Id Details
    2DER  The same protein/tRNA complex in the initial tRNA binding state 
    2DET  The same protein/tRNA complex in the pre-reaction state 
     
     
  •  
    Move Section Ligand Chemical Component Hide
    Identifier Name Formula Interaction View Links
    AMP     ADENOSINE MONOPHOSPHATE C10 H14 N5 O7 P 3DLigand Explorer Link out to Ligand Expos:AMP Link out to SuperLigands:AMP Link out to SuperHapten:AMP
    SO4     SULFATE ION O4 S 3DLigand Explorer Link out to Ligand Expos:SO4 Link out to SuperLigands:SO4 Link out to SuperHapten:SO4
     
     
  •  
    Move Section Derived Data Hide
     
     
 
< Biological Assembly 1    Help >
 
 
 
  •  
    Move Section Deposition Summary Hide
    Authors:   Numata, T.,   Ikeuchi, Y.,   Fukai, S.,   Suzuki, T.,   Nureki, O.

    Deposition:   2006-02-17
    Release:   2006-08-15
    Last Modified (REVDAT):   2009-01-13
     
     
  •  
    Move Section Experimental Details Hide
    Method:   X-RAY DIFFRACTION
    Experimental Data:   Download Structure Factors  [ EDS External Link to EDS ]
    View a histogram of Resolution Resolution[Å]: 3.40
    R-Value: 0.281 (obs.)
    R-Free: 0.318
    Space Group: C 2
    Unit Cell:
      Length [Å] Angles [°]
    a = 238.05 α = 90.00 
    b = 102.13 β = 117.03 
    c = 108.16 γ = 90.00