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CRYSTAL STRUCTURE OF E. COLI 5-METHYLURIDINE METHYLTRANSFERASE RUMA IN COMPLEX WITH RIBOSOMAL RNA SUBSTRATE AND S-ADENOSYLHOMOCYSTEINE.
 
 
DOI:10.2210/pdb2bh2/pdb   NDB ID: 2BH2
2BH2
 
Primary Citation
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  •  
    Move Section Molecular Description Hide
    Classification: Transferase
    Structure Weight: 121465.68
     
    Molecule:23S RRNA (URACIL-5-)-METHYLTRANSFERASE RUMA
    Polymer:1Type:polypeptide(L)Length:433
    Chains:A, B
    EC#:2.1.1.- Go to IUBMB EC entry  
    Other Details:IRON-SULFUR CLUSTER LINKED BY CYS81, CYS87, CYS90, AND CYS162
    Molecule:23S RIBOSOMAL RNA 1932-1968
    Polymer:2Type:polyribonucleotideLength:37
    Chains:C, D
    Other Details:5-FLUORO-U1939 IS METHYLATED AND ITS 6-C IS COVALENTLY LINKED TO CYS389 OF RUMA
     
     
  •  
    Move Section Source Hide
    Polymer: 1
    Scientific Name: Escherichia coli Go to NCBI Taxonomy entry Expression System: Escherichia coli  
    Polymer: 2
    Scientific Name: Synthetic construct Go to NCBI Taxonomy entry  
     
     
  •  
    Move Section Related PDB Entries Hide
    Id Details
    1UWV  CRYSTAL STRUCTURE OF RUMA, THE IRON-SULFUR CLUSTER CONTAINING E. COLI 23S RIBOSOMAL RNA 5-METHYLURIDINE METHYLTRANSFERASE 
     
     
  •  
    Move Section Ligand Chemical Component Hide
    Identifier Name Formula Interaction View Links
    SAH     S-ADENOSYL-L-HOMOCYSTEINE C14 H20 N6 O5 S 3DLigand Explorer Link out to Ligand Expos:SAH Link out to SuperLigands:SAH Link out to SuperHapten:SAH
    SF4     IRON/SULFUR CLUSTER Fe4 S4 3DLigand Explorer Link out to Ligand Expos:SF4 Link out to SuperLigands:SF4 Link out to SuperHapten:SF4
     
     
  •  
    Move Section Modified Residues Hide
    Identifier Name Formula Interaction View Links
    SAH S-ADENOSYL-L-HOMOCYSTEINE (parent: CYS)(type: lPeptideLinking) C14 H20 N6 O5 S 3D Ligand Explorer Link out to Ligand Expos:SAH Link out to SuperLigands:SAH Link out to SuperHapten:SAH 2D
     
     
  •  
    Move Section Derived Data Hide
     
     
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< Biological Assembly 1    Help >
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Biological assembly 1 assigned by authors and generated by PQS (software)
 
 
 
  •  
    Move Section Deposition Summary Hide
    Authors:   Lee, T.T.,   Agarwalla, S.,   Stroud, R.M.

    Deposition:   2005-01-06
    Release:   2005-03-30
    Last Modified (REVDAT):   2009-02-24
     
     
  •  
    Move Section Experimental Details Hide
    Method:   X-RAY DIFFRACTION
    Experimental Data:   N/A
    View a histogram of Resolution Resolution[Å]: 2.15
    R-Value: 0.177 (obs.)
    R-Free: 0.229
    Space Group: C 2
    Unit Cell:
      Length [Å] Angles [°]
    a = 190.06 α = 90.00 
    b = 63.54 β = 125.15 
    c = 112.02 γ = 90.00