Download MendeleyStructure and Function of class-B bacterial non-specific acid phosphatase: functional role of invariant Lys154
Makde, R.D., Gupta, G.D., Kumar, V.To be published.
1Z5U
Crystal structure of S. typhimurium AphA complexed with cyclic-AMP
- PDB DOI: https://doi.org/10.2210/pdb1Z5U/pdb
- Classification: HYDROLASE
- Organism(s): Salmonella enterica subsp. enterica serovar Typhimurium
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2005-03-20 Released: 2006-03-21
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.30 Å
- R-Value Free: 0.204
- R-Value Work: 0.159
This is version 1.4 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| AphA protein | 214 | Salmonella enterica subsp. enterica serovar Typhimurium | Mutation(s): 0 Gene Names: aphA EC: 3.1.3.2 |  | |
UniProt | |||||
Find proteins for Q540U1 (Salmonella typhimurium) Explore Q540U1 Go to UniProtKB: Q540U1 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q540U1 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| CMP Query on CMP | F [auth A], H [auth B], J [auth C], L [auth D] | ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE C10 H12 N5 O6 P IVOMOUWHDPKRLL-KQYNXXCUSA-N |  | ||
| MG Query on MG | E [auth A], G [auth B], I [auth C], K [auth D] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.30 Å
- R-Value Free: 0.204
- R-Value Work: 0.159
- Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 70.71 | α = 90 |
| b = 84.3 | β = 90 |
| c = 149.83 | γ = 90 |
| Software Name | Purpose |
|---|---|
| MOSFLM | data reduction |
| SCALA | data scaling |
| TRUNCATE | data reduction |
| CNS | refinement |
| CCP4 | data scaling |
| TRUNCATE | data scaling |
| CNS | phasing |
Entry History
Deposition Data
- Released Date: 2006-03-21 Deposition Author(s): Makde, R.D., Kumar, V.
Revision History (Full details and data files)
- Version 1.0: 2006-03-21
Type: Initial release - Version 1.1: 2008-04-30
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Version format compliance - Version 1.3: 2017-10-11
Changes: Refinement description - Version 1.4: 2023-10-25
Changes: Data collection, Database references, Derived calculations, Refinement description
