1Z45
Crystal structure of the gal10 fusion protein galactose mutarotase/UDP-galactose 4-epimerase from Saccharomyces cerevisiae complexed with NAD, UDP-glucose, and galactose
- PDB DOI: https://doi.org/10.2210/pdb1Z45/pdb
- Classification: ISOMERASE
- Organism(s): Saccharomyces cerevisiae
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2005-03-15 Released: 2005-04-05
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.85 Å
- R-Value Free: 0.245
- R-Value Work: 0.194
- R-Value Observed: 0.196
This is version 1.7 of the entry. See complete history.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| GAL10 bifunctional protein | 699 | Saccharomyces cerevisiae | Mutation(s): 1 Gene Names: GAL10 EC: 5.1.3.2 (PDB Primary Data), 5.1.3.3 (PDB Primary Data) |  | |
UniProt | |||||
Find proteins for P04397 (Saccharomyces cerevisiae (strain ATCC 204508 / S288c)) Explore P04397 Go to UniProtKB: P04397 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P04397 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 4 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| NAD Query on NAD | E [auth A] | NICOTINAMIDE-ADENINE-DINUCLEOTIDE C21 H27 N7 O14 P2 BAWFJGJZGIEFAR-NNYOXOHSSA-N |  | ||
| UPG Query on UPG | F [auth A] | URIDINE-5'-DIPHOSPHATE-GLUCOSE C15 H24 N2 O17 P2 HSCJRCZFDFQWRP-JZMIEXBBSA-N |  | ||
| GAL Query on GAL | B [auth A] | beta-D-galactopyranose C6 H12 O6 WQZGKKKJIJFFOK-FPRJBGLDSA-N |  | ||
| NA Query on NA | C [auth A], D [auth A] | SODIUM ION Na FKNQFGJONOIPTF-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.85 Å
- R-Value Free: 0.245
- R-Value Work: 0.194
- R-Value Observed: 0.196
- Space Group: I 2 2 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 120.9 | α = 90 |
| b = 125.2 | β = 90 |
| c = 142.3 | γ = 90 |
| Software Name | Purpose |
|---|---|
| PROTEUM PLUS | data collection |
| SAINT | data reduction |
| EPMR | phasing |
| TNT | refinement |
| PROTEUM PLUS | data reduction |
| SAINT | data scaling |
Entry History
Deposition Data
- Released Date: 2005-04-05 Deposition Author(s): Thoden, J.B., Holden, H.M.
Revision History (Full details and data files)
- Version 1.0: 2005-04-05
Type: Initial release - Version 1.1: 2008-04-30
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Version format compliance - Version 1.3: 2012-05-02
Changes: Structure summary - Version 1.4: 2013-05-08
Changes: Non-polymer description - Version 1.5: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Data collection, Derived calculations, Structure summary - Version 1.6: 2021-10-20
Changes: Database references, Structure summary - Version 1.7: 2023-08-23
Changes: Data collection, Refinement description
