1W8N
Contribution of the Active Site Aspartic Acid to Catalysis in the Bacterial Neuraminidase from Micromonospora viridifaciens.
- PDB DOI: https://doi.org/10.2210/pdb1W8N/pdb
- Classification: HYDROLASE
- Organism(s): Micromonospora viridifaciens
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Deposited: 2004-09-24 Released: 2004-09-30
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.10 Å
- R-Value Free: 0.219
- R-Value Work: 0.166
- R-Value Observed: 0.168
This is version 1.4 of the entry. See complete history.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| BACTERIAL SIALIDASE | 601 | Micromonospora viridifaciens | Mutation(s): 1 EC: 3.2.1.18 |  | |
UniProt | |||||
Find proteins for Q02834 (Micromonospora viridifaciens) Explore Q02834 Go to UniProtKB: Q02834 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q02834 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| DAN Query on DAN | C [auth A] | 2-DEOXY-2,3-DEHYDRO-N-ACETYL-NEURAMINIC ACID C11 H17 N O8 JINJZWSZQKHCIP-UFGQHTETSA-N |  | ||
| GAL Query on GAL | B [auth A] | beta-D-galactopyranose C6 H12 O6 WQZGKKKJIJFFOK-FPRJBGLDSA-N |  | ||
| NA Query on NA | D [auth A] | SODIUM ION Na FKNQFGJONOIPTF-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.10 Å
- R-Value Free: 0.219
- R-Value Work: 0.166
- R-Value Observed: 0.168
- Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 46.602 | α = 90 |
| b = 111.608 | β = 90 |
| c = 143.865 | γ = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| MOSFLM | data reduction |
| SCALA | data scaling |
| AMoRE | phasing |
Entry History
Deposition Data
- Released Date: 2004-09-30 Deposition Author(s): Newstead, S., Watson, J.N., Dookhun, V., Bennet, A.J., Taylor, G.
Revision History (Full details and data files)
- Version 1.0: 2004-09-30
Type: Initial release - Version 1.1: 2011-07-13
Changes: Advisory, Version format compliance - Version 1.2: 2017-07-05
Changes: Data collection - Version 1.3: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Data collection, Derived calculations, Other, Structure summary - Version 1.4: 2023-12-13
Changes: Data collection, Database references, Refinement description, Structure summary
